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利用ANS为荧光探剂研究了溴氰菊酯和杀灭菊酯对突触体膜表面性质的影响。这两种带α-氰基的Ⅱ亚型拟除虫菊酯杀虫剂均可进一步增强ANS-突触体膜复合物的荧光强度。双倒数作图分析表明,这一效应是由于膜上ANS 的结合量增加,而不是ANS 的荧光量子产率改变所致。通过Scatchard作图分析测知ANS-突触体膜复合物的结合常数(n,Kd),发现这两种化合物可增加突触体膜对ANS 的结合容量和亲和力,这是膜表面负电荷密度降低的结果。这一效应与溴氰菊酯和杀灭菊酯对膜脂流动性的影响是相关的。
The effect of deltamethrin and fenvalerate on the surface properties of synaptosomal membranes was investigated using ANS as a fluorescent probe. Both of these two α-cyano-containing subtypes of pyrethroid insecticides further enhance the fluorescence intensity of the ANS-synaptosomal membrane complex. Double-reciprocal mapping analysis showed that this effect was due to an increase in the amount of ANS bound on the membrane rather than a change in the fluorescence quantum yield of ANS. The binding constants (n, Kd) of the ANS-synaptosomal membrane complex were measured by Scatchard mapping and found that both compounds increased the binding capacity and affinity of the synaptosomal membrane to ANS, which is the negative charge density on the membrane surface Reduced result. This effect is related to the effect of deltamethrin and fenpropathrin on membrane fluidity.