目的筛选反应停作用于胆囊癌细胞后的相关差异蛋白,探讨反应停对胆囊癌细胞的作用机制。方法应用蛋白质组学技术分离出胆囊癌细胞的差异蛋白,细胞分为实验组和对照组,对两组差异蛋白进行分析和鉴定。结果共发现36个差异在2倍以上的蛋白点(P<0.05)。实验组中13个表达下调,23个上调。选择其中差异在3倍以上的10个蛋白质斑点作为差异表达的质谱分析,鉴定出7个蛋白质点。结论反应停对胆囊癌细胞有一定作用。通过差异蛋白质点进一步探索其对胆囊癌的作用点,可为研究反应停的抗胆囊癌的作用机制提供线索。 Objective To screen the differential proteins involved in the response of gallbladder carcinoma cells and to explore the mechanism of action of haloderate on gallbladder carcinoma cells. Methods The differential proteins of gallbladder carcinoma cells were isolated by proteomic techniques. The cells were divided into experimental group and control group, and the differential proteins of two groups were analyzed and identified. Results A total of 36 spots with more than 2 folds were found (P <0.05). In the experimental group, 13 were down-regulated and 23 were up-regulated. Seven protein spots were identified by mass spectrometry analysis that selected 10 protein spots where the difference was more than 3-fold as a differential expression. Conclusion The response to gallbladder cancer cells have a role. To further explore its role in gallbladder cancer by differentially expressed protein spots can provide clues for studying the mechanism of anti-gallbladder cancer response.