维生素A是儿童容易缺乏的一种营养素。目前我国调查城市儿童A摄入量的较多,检测其血清维生素A含量的较少。为此,我们对南宁市某幼儿园儿童进行了膳食中维生素A及胡萝卜素摄入量、血清维生素A含量及暗适应时的调查与测定。对象1992年11月于市立某幼儿园日托的4～6岁健康儿童中,选出自愿且未服用过维生素A制剂者50名为调查对象。一般情况及膳食调查用统一表格以询问法进行,膳令数据用微机统计。血清维生素A测定抽静脉血,血清以正己烷提取,用岛津高效液相构谱仪测定,层析柱为:ZORBAX-ODSC_18反相柱,流动相为甲醇,流量为1ml/min,紫外 Vitamin A is a nutrient that children easily lack. At present, China’s urban children’s A intake survey more, less detection of serum vitamin A content. To this end, we conducted a survey and determination of dietary vitamin A and carotenoid intake, serum vitamin A content and dark adaptation in a nursery school in Nanning. Object In November 1992 in a municipal nursery day care of 4 to 6-year-old healthy children, selected voluntary and did not take vitamin A preparations were 50 were surveyed. The general situation and dietary survey using a unified form to ask law, meal data using computer statistics. Serum vitamin A determination of venous blood, the serum extracted with n-hexane, with Shimadzu high performance liquid chromatography, chromatography column: ZORBAX-ODSC_18 reverse phase column, the mobile phase of methanol, the flow rate of 1ml / min, UV