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以化学诱变剂甲基磺酸乙酯(EMS)和香蕉枯萎病菌(Fusarium oxysporum f.sp.cubense)4号生理小种的粗毒素为诱变剂,以香蕉愈伤组织和分化芽为筛选材料,通过多步筛选法,优化了筛选体系,并筛选出了抗香蕉枯萎病菌毒素的突变体。以体积百分比浓度为0.5%的EMS分别处理香蕉愈伤组织和分化芽40min,获得“半致死剂量”效应,对存活的香蕉愈伤组织和分化芽在依次递增的粗毒素浓度(5.0%→10.0%→12.5%)胁迫下进行定向筛选,获得了抗香蕉枯萎病菌毒素的愈伤组织块和分化芽,从抗病分化芽突变体获得了再生植株。对再生植株抗毒素的性能进行鉴定,其病情指数(24.72)显著低于对照植株(96.11),抗毒素效果达到74.28%。
The crude mutants of chemical racemization ethylmethylsulfonate (EMS) and Fusarium oxysporum f.sp.cubense (No.4) were used as mutagens, and the callus of banana and buds were screened Materials, through a multi-step screening method, the screening system was optimized, and mutants resistant to Fusarium oxysporum fusca were screened out. The banana callus and buds were treated with 0.5% EMS at 40 min respectively to obtain the “semi-lethal dose” effect. The surviving banana callus and buds were treated with increasing concentrations of crude toxin (5.0% → 10.0% → 12.5%), the callus blocks and differentiated buds of Fusarium oxysporum f. Sp. Isolates were obtained, and the regenerated plants were obtained from the mutants of disease-resistant differentiation buds. The performance of the regenerated plant antitoxin was identified. The disease index (24.72) was significantly lower than that of the control plant (96.11), and the antitoxin effect reached 74.28%.