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目的:通过测定子痫前期(PE)患者胎盘组织和血清中白细胞介素-18(IL-18)和人类软骨糖蛋白39(YKL-40)的表达情况,分析两者之间的相关性,进而为阐明子痫前期的发病机理提供理论依据。方法:采用免疫组化两步法和酶联免疫吸附试验分别检测20例正常孕妇(对照组)和50例子痫前期患者(实验组)胎盘组织和血清中IL-18和YKL-40的表达水平。其中轻度子痫前期20例(MPE),重度子痫前期30例(SPE)。结果:实验组胎盘组织中IL-18表达水平显著高于对照组(P<0.01);SPE组胎盘组织中IL-18的表达水平显著高于MPE组(P<0.01)。实验组胎盘组织中YKL-40表达水平显著高于对照组(P<0.05);SPE组胎盘组织中YKL-40的表达水平明显高于MPE组(P<0.05)。通过酶联免疫吸附试验(ELISA)看出,实验组血清中的IL-18和YKL-40的表达水平均显著高于对照组(P<0.01),SPE患者血清中YKL-40及IL-18的水平显著高于MPE组(P<0.05)。实验组胎盘组织和血清中IL-18和YKL-40的表达分别显示两者呈正相关(P<0.01)。结论:子痫前期患者胎盘组织和血清中IL-18及YKL-40表达水平均明显高于正常对照组,与子痫前期病情严重程度明显相关,且两者之间均呈正相关,可能参与了子痫前期的发病过程。
OBJECTIVE: To determine the correlation between the expression of interleukin-18 (IL-18) and human chondrogenesis-glycoprotein 39 (YKL-40) in placenta tissue and serum of patients with preeclampsia (PE) In order to elucidate the pathogenesis of preeclampsia provide a theoretical basis. Methods: The expression of IL-18 and YKL-40 in placenta tissue and serum of 20 normal pregnant women (control group) and 50 preeclampsia patients (experimental group) were detected by immunohistochemical two-step method and enzyme-linked immunosorbent assay . Among them, 20 were mild preeclampsia (MPE) and 30 were severe preeclampsia (SPE). Results: The level of IL-18 in placenta of experimental group was significantly higher than that in control group (P <0.01). The level of IL-18 in placenta of SPE group was significantly higher than that of MPE group (P <0.01). The level of YKL-40 in placenta of experimental group was significantly higher than that in control group (P <0.05). The expression level of YKL-40 in placenta of SPE group was significantly higher than that of MPE group (P <0.05). The levels of IL-18 and YKL-40 in the serum of the experimental group were significantly higher than those of the control group (P <0.01) by enzyme linked immunosorbent assay (ELISA). The levels of YKL-40 and IL-18 Was significantly higher than that of MPE group (P <0.05). The expression of IL-18 and YKL-40 in the placenta and serum of the experimental group showed a positive correlation (P <0.01). Conclusion: The expression levels of IL-18 and YKL-40 in placenta tissue and serum of preeclampsia patients were significantly higher than those in normal control group, which were significantly correlated with the severity of preeclampsia, and both of them were positively correlated and may be involved in The pathogenesis of preeclampsia.