论文部分内容阅读
恶性肿瘤细胞的核仁不仅在形态学上,而且在免疫反应性上也发生改变,出现特殊的肿瘤核仁抗原——人恶性肿瘤相关核仁抗原(HMNA)。为寻找新的、有癌症诊断意义的HMNA,本研究以A549细胞和人肺腺癌细胞核仁为抗原,建立了7株鼠抗人癌细胞核仁抗原单克隆抗体(MA_(1~6)和ML_1),并用ELISA、间接免疫荧光(IF)、免疫印渍、免疫电镜、细胞同步化培养、细胞分化的诱导和IF显微分光光
The nucleoli of malignant tumor cells not only morphologically but also immunoreactively changes, resulting in the appearance of a specific tumor nucleolar antigen, the human malignant tumor-associated nucleolar antigen (HMNA). In order to search for new HMNAs with cancer diagnosis, we established 7 mouse anti-human cancer cell nucleolar antigen monoclonal antibodies (MA_(1~6) and ML_1) using A549 cells and human lung adenocarcinoma nucleoli as antigens. ), and use ELISA, indirect immunofluorescence (IF), immunoblotting, immunoelectron microscopy, cell synchronization culture, induction of cell differentiation, and IF spectroscopic light