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本研究以47份经甄别鉴定的宁夏水稻品种(系)为试验材料,筛选出28对较均匀分布于12条染色体、条带清晰稳定、多态性丰富的SSR标记构建了宁夏水稻微卫星标记数据库。共检测到144个等位基因,标记间差异性位点数3~10个,平均5.14个;平均Nei’s遗传多样性指数为0.6187。28个位点上参试材料间均有差异。只在1个位点上存在差异的是宁粳28号和宁粳23号,宁粳23和宁粳35号;其余品种差异性位点均在2个或2个以上,占参试材料的93.6%。28对引物中筛选出10对核心引物,各材料间至少有1对引物存在差异,能够把参试品种(系)一一区分开来。利用这10对引物,在相同的迁移位置上以1、0标记扩增片段的有无,构建了宁夏水稻DNA指纹图谱。
In this study, 47 japonica rice varieties (lines) which were screened and identified were used as materials to screen 28 pairs of SSR markers that were evenly distributed on 12 chromosomes with clear and stable bands and abundant polymorphism, and constructed Ningxia rice microsatellite markers database. A total of 144 alleles were detected with 3 to 10 differential loci with an average of 5.14. The average Nei’s genetic diversity index was 0.6187. There were differences among the tested materials on the eight loci. Only one locus of difference is Ningjing 28 and Ningjing 23, Ningjing 23 and Ningjing 35; the remaining varieties are more than 2 or more sites, accounting for the test material 93.6%. 28 pairs of primers were screened out of 10 pairs of core primers, at least one pair of primers between the different materials, the test varieties (lines) one by one to distinguish. The 10 pairs of primers were used to construct DNA fingerprinting of Ningxia rice at the same migration position with 1, 0 mark amplification fragments.