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目的 :研究心肌缺血预适应时 ,腺苷酸环化酶 (AC)及cAMP变化及意义。方法 :选择SD大鼠 ,随机分成预适应组 (IP组 ) ,缺血 /再灌注组 (I/R组 )及假手术组 (CON组 ) ,手术套管法造成左冠脉主干缺血及再灌注。损伤后取心脏用TTC法测梗塞面积 ,取血清测心肌酶改变 ,测AC活性变化 ,放免测cAMP水平。结果 :IP组较I/R组心梗面积明显缩小 (p <0 0 0 1) ,心肌酶CK、CK—MB、LDH明显降低 (p <0 0 0 1)。IP组较I/R组AC活性增高 (p<0 0 5 ) ,cAMP含量增加 (p<0 0 0 1)。结论 :心肌缺血预适应保护心脏 ,缩小梗塞面积 ,减少心肌酶漏出 ,cAMP可能在预适应保护作用中起重要作用。
Objective: To study the changes and significance of adenylate cyclase (AC) and cAMP in myocardial ischemic preconditioning. Methods: Sprague - Dawley rats were randomly divided into three groups: preconditioning group (IP group), ischemia / reperfusion group (I / R group) and sham operation group (CON group) Reperfusion. Take the heart after injury TTC method to measure the infarction area, take serum changes of myocardial enzymes, measured changes in AC activity, radiotherapy-free cAMP levels. Results: Compared with the I / R group, the myocardial infarct size in IP group was significantly reduced (p <0.01 01), and CK, CK-MB and LDH were significantly decreased (p <0.01 01). Compared with the I / R group, the IP group had higher AC activity (p <0 05) and cAMP content increased (p 0 001). Conclusion: Myocardial ischemic preconditioning protects the heart, reduces infarct size and reduces myocardial enzyme leakage. CAMP may play an important role in the protective effect of preconditioning.