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目的探讨二甲基甲酰胺(dimethylformamide,DMF)致H9c2心肌细胞损伤及维生素C(vitamin C,VC)对此损伤是否有保护作用。方法观察DMF染毒及VC干预后心肌细胞及核形态改变;活性氧(reactive oxygen species,ROS)、总抗氧化能力(total antioxidant capacity,T-AOC)试剂盒检测不同浓度DMF染毒不同时间、特定浓度DMF合并不同浓度VC干预后细胞氧化应激水平变化。结果 DMF染毒后细胞及核形态出现不同程度异常,低浓度VC干预后有所改善;100、140、180、220、250 m M DMF染毒24 h,100、140、180、200、220 m M DMF染毒48 h,100、125、140、180、200 m M DMF染毒72 h后,同时间不同剂量染毒,细胞ROS、T-AOC水平差异有统计学意义;同剂量不同时间染毒,细胞ROS、T-AOC水平差异有统计学意义(ROS:24 h:F=5 763.00,P<0.001;48 h:F=5 861.00,P<0.001;72 h:F=9 188.00,P<0.001;T-AOC:24 h:F=6.25,P=0.004;48 h:F=11.48,P<0.001;72 h:F=14.13,P<0.001)。VC(0.025、0.05、0.10、0.25 m M)干预后ROS、T-AOC水平与对照相比差异均有统计学意义(均有P<0.05)。结论氧化损伤可能是DMF发挥毒性的重要机制;低剂量VC可缓解DMF产生的氧化损伤,对细胞产生保护效应。
Objective To investigate whether H9c2-induced cardiomyocyte injury caused by dimethylformamide (DMF) and vitamin C (VC) have a protective effect on this injury. Methods The morphological changes of myocardial cells and nuclei were observed after exposure to DMF and VC. The levels of reactive oxygen species (ROS) and total antioxidant capacity (T-AOC) were measured at different time points. Changes of oxidative stress in cells exposed to specific concentration of DMF combined with different concentration of. Results The morphological changes of cells and nuclei were different after the exposure to DMF. The concentrations of VC and VC were improved after low-concentration VC treatment. The concentrations of 100, 140, 180, 220 and 250 m M DMF for 24 h, 100, 140, 180, 200 and 220 m M DMF for 48 h, 100, 125, 140, 180 and 200 m M DMF for 72 h, the same time different doses of exposure, cell ROS, T-AOC levels were significantly different; The levels of ROS, T-AOC in cells were significantly higher than those in control group (ROS: 24 h: F = 5 763.00, P <0.001; 48 h: F = 5 861.00, P <0.001; 72 h: F = <0.001; T-AOC: 24 h: F = 6.25, P = 0.004; 48 h: F = 11.48, P <0.001; 72 h: F = 14.13, P <0.001). The levels of ROS and T-AOC in VC (0.025,0.05,0.10,0.25 m M) intervention group were significantly different from those in control group (all P <0.05). Conclusion Oxidative damage may play an important role in the toxicity of DMF. Low doses of VC can alleviate the oxidative damage caused by DMF and have a protective effect on the cells.