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高分子量谷蛋白亚基(HMW-GS)对小麦面粉加工品质有促进作用,尤其是Glu-D1d基因编码的1Dx5+1Dy10亚基能增加面团的筋度和弹性。小麦背景中的1BL.1RS易位对小麦面粉加工品质有显著的负面影响。因此,在小麦品质育种中如何判定小麦背景中是否含有1BL.1RS易位和HMW-GS的Glu-D1d基因具有重要意义。本研究利用3对分别检测1BL.1RS易位、Glu-B3和Glu-D1位点的共显性特异标记,结合SDS-PAGE鉴定,对16份已知遗传背景和Glu-D1x等位基因材料及38株(周麦18×烟农19)F2群体进行了分析,探索出适合同时鉴定小麦背景中1BL.1RS易位和Glu-D1d基因的多重PCR技术实验体系,并采用该体系对国内外352份小麦品种(系)进行了鉴定。结果表明,该体系是同时鉴定小麦背景中1BL.1RS易位和Glu-D1d基因的一种非常有效、简便可行的实验方法,可在标记辅助选择(MAS)育种中应用。
High-molecular-weight glutenin subunit (HMW-GS) can promote wheat flour processing quality, especially the 1Dx5 + 1Dy10 subunit encoded by Glu-D1d gene can increase the strength and elasticity of dough. The 1BL.1RS translocation in the wheat background has a significant negative impact on wheat flour processing quality. Therefore, how to determine whether the wheat background contains the 1BL.1RS translocation and the Glu-D1d gene of HMW-GS in wheat quality breeding is of great significance. In this study, we detected the co-dominant specific markers of 1BL.1RS translocation, Glu-B3 and Glu-D1 loci by three pairs of SDS-PAGE and 16 pairs of known genetic background and Glu-D1x alleles And 38 F2 populations (Zhoumai 18 × Yannong 19) were analyzed, and the experimental system suitable for the simultaneous identification of 1BL.1RS translocation and Glu-D1d gene in wheat background was explored. Wheat varieties (lines) were identified. The results showed that this system is a very effective, simple and feasible experimental method for the simultaneous identification of 1BL.1RS translocation and Glu-D1d gene in wheat background. It can be used in marker-assisted selection (MAS) breeding.