论文部分内容阅读
目的分析1999～2003年中国(未包括香港、澳门特别行政区和台湾地区,下同)流行的H1基因型麻疹野病毒代表株血溶素蛋白(Fusion Protein,F)基因的分子特征。方法从1999～2003年中国分离的麻疹野病毒株中,选取13株H1基因型代表株,包括H1a基因亚型麻疹野病毒8株和H1b基因亚型麻疹野病毒5株。使用逆转录-聚合酶链反应扩增病毒的F基因全长,并进行核苷酸序列测定,同时与中国疫苗株及其它国家的A、D3、D6、D7、E基因型参考株进行序列比对及基因亲缘性关系分析。结果1999～2003年13株中国流行麻疹野病毒代表株中,核苷酸同源性为98.1%～99.0%,氨基酸同源性为98.1%～100.0%,与中国疫苗株沪191相比,其核苷酸同源性为95.7%～96.2%,氨基酸同源性为96.9%～97.2%;而与其它基因型相比,核苷酸同源性为94.4%～96.2%。F基因3个糖基化位点(第32、64、70位氨基酸)、对病毒融合功能起着重要作用的第112位精氨酸、第195位亮氨酸未发生改变。结论1999～2003年中国流行的H1基因型麻疹野病毒的F基因无明显差异,F蛋白上已知的重要功能位点未发生变异,推测中国流行的麻疹野病毒F蛋白的结构和功能未发生较大改变,但由于F蛋白是重要的功能蛋白,对F蛋白核苷酸和氨基酸的变异规律进行常规监测,对了解麻疹野病毒流行特点及其遗传变异规律具有重要意义。
Objective To analyze the molecular features of Fusion Protein (F) gene of representative H1NFV in China from 1999 to 2003 (excluding Hong Kong, Macao Special Administrative Region and Taiwan). Methods Thirteen H1 genotypes were selected from wild measles virus isolates isolated in China from 1999 to 2003, including 8 H1a subtype measles virus and 5 H1N1 subtype measles virus. The full-length F gene of the virus was amplified by reverse transcription-polymerase chain reaction (PCR) and the nucleotide sequence was determined. At the same time, it was compared with Chinese vaccine strains and reference strains A, D3, D6, D7 and E in other countries Analysis of the relationship with gene affinity. Results Among the 13 strains of wild measles virus in China from 1999 to 2003, the nucleotide homology was 98.1% -99.0% and the amino acid homology was 98.1% -100.0%. Compared with the Chinese vaccine strain Shanghai 191 Nucleotide homology was 95.7% ~ 96.2%, and amino acid homology was 96.9% ~ 97.2%. Compared with other genotypes, nucleotide homology was 94.4% ~ 96.2%. Three glycosylation sites (amino acids 32, 64, and 70) of F gene, the 112th arginine which plays an important role in viral fusion function, and the 195th leucine did not change. Conclusion There was no significant difference in the F gene of the H1N1 measles wild virus in China from 1999 to 2003, and no significant changes in the known function of the F protein were observed. It is speculated that the structure and function of the wild F protein of the wild measles virus in China did not occur However, since F protein is an important functional protein, routine monitoring of the variation of nucleotide and amino acid of F protein is of great significance to understand the epidemiological characteristics and genetic variation of wild measles virus.