本文报道了用反相高效液相色谱法（ＲＰＨＰＬＣ）同时高效、快速分离和测定极性及非极性芳香化合物。在ＯＤＳ柱上，以甲醇、水、四氢呋喃作流动相，采用梯度淋洗法，在１２ｍｉｎ内定量分离了邻－、间－、对－苯二酚、苯酚及联苯混合物。详细研究了流动相组成和淋洗方法对化合物色谱保留行为的影响，并应用于苯催化氧化反应产物的分析。结果表明正铜盐复合型催化剂（ＣｕＣｌ２＋漆酚树脂）是文中所研究的催化剂中是效率较高，选择性催化好的一种催化剂。 This paper reports the simultaneous efficient and rapid separation and determination of polar and non-polar aromatics using reversed-phase high performance liquid chromatography (RPHPLC). On ODS column, methanol, water and tetrahydrofuran were used as mobile phase, and the mixture of o-, m-, p-hydroquinone, phenol and biphenyl was quantitatively separated within 12 min by gradient elution. The influences of mobile phase composition and elution method on the chromatographic retention behavior of the compounds were studied in detail and applied to the analysis of the products of benzene oxidation reaction. The results show that CuCl2 + urushiol resin is one of the most efficient and selective catalysts in the study.