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介绍一种从不同类型细胞或不同生长状态细胞中分离差异表达基因的快速ｍＲＮＡ差异显示技术．其特点是不用同位素标记，操作简便，在普通琼脂糖凝胶电泳中就能分辨差异显示的ｃＤＮＡ带，便于ＤＮＡ回收和进一步重组克隆．用此方法成功地分离到电离辐射诱导转录子．
This paper introduces a rapid mRNA differential display technique for separating differentially expressed genes from different types of cells or cells of different growth states. Its characteristic is not labeled with isotope, easy to operate, in the ordinary agarose gel electrophoresis will be able to distinguish the difference between the displayed cDNA bands for DNA recovery and further recombinant cloning. Ionizing radiation-induced transcripts were successfully isolated by this method.