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合成乳糖酰基壳聚糖(GC)并以此为载体材料,采用静电喷射法制备载白介素1受体拮抗剂(IL-1Ra)GC纳米颗粒.在实验过程中,加入一定量聚氧化乙烯(PEO)于GC电纺溶液中,考察电纺溶液性质对颗粒形成的影响,优化溶液性质参数,通过扫描电子显微镜(SEM)观察得到表面光滑、颗粒平均直径约为530nm的载IL-1Ra纳米颗粒,并通过酶联免疫(ELISA)试剂盒检测得到载药纳米颗粒包封率为(1.52±0.04)%(n=3),载药率达到(90.36±3.46)%(n=3).为了考察所制备的GC纳米颗粒对肝细胞的亲和靶向性,实验以猪肝细胞为实验组,猪骨髓基质干细胞为实验对照组,分别加入FITC标记的GC纳米颗粒培养24h,荧光显微镜下观察到猪肝细胞表面荧光信号明显强于骨髓基质干细胞,表明本实验所制备的GC纳米颗粒具有明显的肝靶向功能.
(IL-1Ra) GC nanoparticles were prepared by electrospray method using lactose acyl chitosan (GC) as the carrier material.In the experiment, a certain amount of polyethylene oxide (PEO ) In GC electrospinning solution, the influence of the properties of electrospinning solution on the formation of particles was investigated, the parameters of the solution were optimized, the IL-1Ra nanoparticles with smooth surface and average diameter of about 530nm were observed by scanning electron microscope (SEM) The encapsulation efficiency of drug-loaded nanoparticles was (1.52 ± 0.04)% (n = 3) and the drug-loading rate was (90.36 ± 3.46)% (n = 3) by enzyme-linked immunosorbent assay (ELISA) The prepared GC nanoparticles were used for the affinity targeting of hepatocytes. The experimental group consisted of porcine hepatocytes and porcine bone marrow stromal cells as experimental control group. FITC-labeled GC nanoparticles were added respectively and cultured for 24 hours. The results were observed under a fluorescence microscope The fluorescence signal on the surface of porcine hepatocytes was significantly stronger than that of bone marrow stromal cells, indicating that the GC nanoparticles prepared in this experiment have obvious liver-targeting function.