目的探讨保存pTAT-HA质粒转化菌简单有效的方法。方法配制LB细菌培养基,将pTAT-HA质粒转化至DH5α感受态细胞,筛选成功转化子并扩增培养,将转化菌接种在不同浓度甘油菌液和新鲜牛奶中,-70℃保存6个月和1年后分别复苏,计算存活率,分析保存效果。结果保存6个月以30%甘油、40%甘油和新鲜牛奶保存效果较好,保存1年仅见30%甘油、40%甘油和新鲜牛奶冻存管有存活菌。结论pTAT-HA质粒转化菌宜使用30%～40%甘油菌液或新鲜牛奶保存。 Objective To explore a simple and effective method for preserving pTAT-HA plasmid-transformed bacteria. Methods The LB bacterial culture medium was prepared. The pTAT-HA plasmid was transformed into DH5α competent cells, and the transformants were screened and amplified. The transformed bacteria were inoculated into different concentrations of glycerol and fresh milk and stored at -70 ℃ for 6 months And 1 year after recovery, calculate the survival rate, analysis of the preservation effect. Results The preservation for 6 months was better with 30% glycerol, 40% glycerol and fresh milk. Only 30% glycerol and 40% glycerol and fresh milk were stored for 1 year. Conclusion pTAT-HA plasmid transformed bacteria should use 30% ~ 40% glycerol solution or fresh milk preservation.