论文部分内容阅读
主要研究了乙酰胆碱酯酶的提取、底物与酶量的关系及几种化学农药对乙酰胆碱酯酶的抑制作用 ;确定水解 1ml1mmol/L底物的最适酶量及几种化学农药对乙酰胆碱酯酶的Ⅰ50 值 ;确定Asch -DTNB法是一种检测果蔬中化学农药残留的快速简便方法 ,并试制出快速检测卡 ,其检测灵敏度达 0 0 5mg/L。
Mainly studied the extraction of acetylcholinesterase, the relationship between the substrate and the amount of enzyme and several chemical pesticides on the inhibition of acetylcholinesterase; determine the hydrolysis of 1ml1mmol / L substrate optimum amount of enzyme and several chemical pesticides on acetylcholinesterase The value of Ⅰ50 was determined. Asch-DTNB method was a quick and easy method to detect chemical pesticide residues in fruits and vegetables. The rapid test card was developed with a detection sensitivity of 0 0 5 mg / L.