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随着结核菌痰检质控的开展,以及在防痨工作中要求基层实验室及查痰点将痰涂片保存半年或半年以上,留待上级结防机构复查。在复查过程中,有时需要对不符合要求或保存时间长结核菌染色已褪的痰涂片进行重染。目前实验室多采用脱色剂脱色后重染的方法,本实验方法不需先行脱色,直接将已染过的痰涂片重染,现报道如下。材料与方法对保存于标本盒内的1988年3～11月间经抗酸染色油镜检查为阳性的门诊病人痰涂片进行复查,观察全片仍能找到结核菌者为阳性,因染色已褪而未能找到结核菌者则进行重染。每片至少镜检100个视野或不少于5分钟。从保存时间达6个月以上,而未能找到结核菌的涂片中,抽取8份采用直接重染的方法查结核菌,结果见附表。
With the tuberculosis sputum quality control carried out, and in the anti-tuberculosis work required the grassroots laboratories and sputum sputum smear stored for six months or more than half, leaving the higher level of anti-TB agency review. In the review process, sometimes need to do not meet the requirements or save a long time stained Mycobacterium tuberculosis faded smear for heavy dye. At present, many laboratories use decolorizing agent decolorant after dyeing method, the experimental method without prior decolorization, directly stained the smear has been stained, are reported below. Materials and Methods The sputum smear of outpatients who were positive by acid-fast staining for gastroscopy during the period from March to November, 1988, which was stored in the specimen box, was reviewed. The results showed that the whole film was still positive for finding TB, Tainted and failed to find those who carry heavy stains TB. Each piece at least microscopic examination of 100 fields or not less than 5 minutes. From the preservation time of 6 months or more, but failed to find the smear of TB, extract 8 copies of the method of direct staining of tuberculosis, the results see Schedule.