论文部分内容阅读
目的:筛查大肠癌分化相关分子标志物。方法:应用美国A ffym etrix HG-U133寡核苷酸芯片对不同分化程度大肠癌组织和正常大肠黏膜组织的基因表达谱进行检测。采用肿瘤样品分类法(T-c lass)对各样品进行基因分类。并应用实时荧光定量RT-PCR检测候选基因在不同分化程度大肠癌中的差异表达。结果:T-c lass分析获得分类精度100%的大肠癌分化差异表达基因&ESTs 8个。其中C8orf4基因(chromosom e 8 open read ing fram e4)经非多元统计分析,在大肠癌组较正常黏膜表达下调。实时荧光定量RT-PCR检测该基因在不同分化程度大肠癌组间表达,其标准化表达值与基因芯片该基因探针组信号值相关性分析,呈线性相关。两种方法检测C8orf4基因在不同分化程度大肠癌组表达趋势相符。结论:C8orf4基因与大肠癌分化密切相关,可作为大肠癌分化的候选分子标志物。
Objective: To screen molecular marker of differentiation of colorectal cancer. Methods: The gene expression profiles of colorectal cancer tissues and normal colorectal mucosa tissues with different differentiation degrees were detected by the American A ffym etrix HG-U133 oligonucleotide microarray. Each sample was genotyped using T-c lass. Real-time fluorescent quantitative RT-PCR was used to detect the differential expression of candidate genes in different degree of differentiation of colorectal cancer. Results: Eight differentially expressed genes & ESTs of colorectal cancer with classification accuracy of 100% were obtained by T-c lass analysis. Among them, C8orf4 gene (chromosom e8 open read in fram e4) by non-multivariate statistical analysis, colorectal cancer group than normal mucosa down regulation. Real-time fluorescent quantitative RT-PCR was used to detect the expression of this gene in colorectal cancer tissues with different differentiation degrees. The correlation between the normalized expression value and the signal value of this gene probe group was linearly correlated. Two methods to detect C8orf4 gene expression in different degrees of differentiation of colorectal cancer consistent with the trend. Conclusion: The gene of C8orf4 is closely related to the differentiation of colorectal cancer and may be used as a candidate molecular marker for the differentiation of colorectal cancer.