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目的:优选兴安升麻地上部分总皂苷的提取分离工艺。方法:以总皂苷含量为指标,采用分段萃取法用不同试剂萃取升麻地上部分中的有效成分,旋转蒸发回收溶剂,将正丁醇萃取物通过大孔吸附树脂柱进一步分离,并通过紫外分光光度计进行定量检测。结果:采用正丁醇萃取后得到的物质过大孔吸附树脂后,总皂苷纯度>50%。结论:通过萃取将升麻地上部分进一步分离,把有效成分按极性分段,便于后期对兴安升麻地上部分总皂苷进一步分离纯化,同时能得到较高纯度的总皂苷。
Objective: To optimize the extraction and separation of total saponins from the top of Xing’an Cimicifugae. Methods: The total saponin content as an indicator, the use of different parts of the extraction method with different components of the aerial parts of Cimicifuga fraction recovery solvent, the n-butanol extract through the macroporous resin column for further separation by UV Spectrophotometer for quantitative detection. Results: After the macroporous resin was obtained from n-butanol extraction, the total saponin purity was> 50%. CONCLUSION: The above-ground part of Cimicifuga can be further separated by extraction, and the active ingredients are segmented according to the polarity so as to facilitate the further separation and purification of the above-ground part of gypenosides, and at the same time, the higher purity of total saponin can be obtained.