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萜类是植物生长发育中具有重要作用的次级代谢产物,某些萜类可被生物和非生物胁迫诱导产生。用200 mmol/L Na Cl溶液处理商用玉米品种天塔五号(F1)及其父母本三叶期幼苗,qRT-PCR结果发现萜类合成途径中的萜烯合酶基因2(terpene synthase 2,TPS2)、萜烯合酶基因3(terpene synthase 3,TPS3)、牻牛儿基牻牛儿基焦磷酸合成酶基因4(geranylgeranyl diphosphate synthase 4,GGPS4)在三组材料中表达量随胁迫时间延长均先上调再下调,且F1表达量显著高于亲本。F1自交F2代耐盐性及基因表达情况分析表明,F2代中三个基因表达仍与耐盐性呈正相关。对盐胁迫条件下总类胡萝卜素及各成分含量、生物量、光合指标、叶绿素和脯氨酸含量进行测定,发现F1耐盐能力明显优于亲本,表明TPS2、TPS3、GGPS4基因高水平表达与F1发挥耐盐优势、抵御逆境胁迫有一定相关性。
Terpenoids are secondary metabolites that play an important role in plant growth and development. Some terpenoids can be induced by biotic and abiotic stresses. The results of qRT-PCR showed that the terpene synthase 2 (terpene synthase 2) in commercial corn cultivar Tian Tap No.5 (F1) and its parental trilobe seedling were treated with 200 mmol / L NaCl solution. TPS2), terpene synthase 3 (TPS3) and geranylgeranyl diphosphate synthase 4 (GGPS4) in the three groups of materials with the prolongation of stress time Both first and then down regulation, and F1 expression was significantly higher than the parental. Analysis of salt tolerance and gene expression in F1 selfed F2 progeny indicated that the expression of three genes in F2 progeny was still positively correlated with salt tolerance. The contents of total carotenoids and their components, biomass, photosynthetic indexes, chlorophyll and proline contents were measured under salt stress. It was found that the salt tolerant ability of F1 was significantly better than that of parents, indicating that the high level expression of TPS2, TPS3 and GGPS4 F1 to play a salt-resistant advantages, to resist stress has some relevance.