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[目的]观察软癥散结合剂对裸鼠人食管癌移植瘤的抑制作用,及其对瘤体BAT-26及D3S1067微卫星不稳定性(MSI)的影响。[方法]建立裸鼠皮下食管癌移植瘤模型,模型建成后随机分为5组:正常组、生理盐水组、软癥散结合剂低、中、高剂量组。实验15d后剥取肿瘤并称重,计算抑瘤率。荧光PCR法检测移植瘤组织中BAT-26及D3S1067MSI的表达。[结果]低、中、高剂量软癥散结合剂对裸鼠的移植瘤抑制率分别为27.11%、47.69%和35.56%。软癥散结合剂中剂量组D3S1067 MSI发生率显著性低于生理盐水组(P<0.05)。各组BAT-26位点MSI表达均很低,且无统计学差异。[结论]软癥散结合剂能抑制人食管癌细胞移植瘤生长,这一作用可能与其下调肿瘤细胞D3S1067位点的微卫星不稳定性相关。
[Objective] To observe the inhibitory effect of “Fuzheng Sanjie Decoction” on human esophageal carcinoma in nude mice and its effect on microsatellite instability (MSI) of tumor cells BAT-26 and D3S1067. [Method] The model of transplanted esophageal cancer in nude mice was established. The model was randomly divided into 5 groups: normal group, normal saline group, low, medium and high dose group of soft dispersible binder. After 15 days, the tumors were stripped and weighed to calculate the tumor inhibition rate. Fluorescent PCR was used to detect the expression of BAT-26 and D3S1067 MSI in the xenografts. [Results] The inhibitory rates of low, medium and high doses of soft dispersible combination on nude mice were 27.11%, 47.69% and 35.56%, respectively. The incidence of MSS D3S1067 was significantly lower in the moderate-dose group than in the NS group (P <0.05). The expression of MSI in BAT-26 loci was very low in all groups, with no significant difference. [Conclusion] SFS can inhibit the growth of human esophageal cancer xenografts, which may be related to the down-regulation of the microsatellite instability of D3S1067 tumor cells.