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用负向选择增富法成功地获得人GM-CSF cDNA克隆,筛选阳性率提高至1/170。用该cDNA转染的CDS-7细胞上清可测出集落刺激活性。经亚克隆化后在大肠杆菌表达出重组人GMCSF,可维持细胞株BMU-2的生长并促进急性非淋巴细胞白血病(ANLL)患者骨髓细胞的集落形成。
The human GM-CSF cDNA clone was successfully obtained by the negative selection enrichment method and the screening positive rate was increased to 1/170. Colony-stimulating activity was measured on the CDS-7 cell supernatant transfected with this cDNA. Recombinant human GMCSF was expressed in E. coli after subcloning, which can maintain the growth of BMU-2 cell line and promote the formation of bone marrow cells in patients with acute non-lymphocytic leukemia (ANLL).