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目的观察Ｒｂ蛋白在胃癌中的表达及与浸润、转移和临床分期的关系。方法用链霉菌抗生物素蛋白—过氧化酶连接法检测Ｒｂ蛋白在正常胃粘膜、不典型增生、胃癌中的表达。结果Ｒｂ蛋白表达率，正常胃粘膜１００％（１０／１０），明显高于不典型增生８０％（４８／６０）（Ｐ＜０．０５）和胃癌６０％（７２／１２０）（Ｐ＜０．０１）；不典型增生与胃癌有显著性差异（Ｐ＜０．０１），但中度８０％（１６／２０）、重度７５％（１２／２０）却无显著性；高分化腺癌６８％（２３／３４）、低分化腺癌５８％（２９／５０）、未分化癌６４％（９／１４）、粘液癌５０％（６／１２）、印戒细胞癌５０％（５／１０），各型之间无显著差异；浸润浅肌层者７５％（３０／４８）较浸润深肌层及全层者５０％（３６／７２）（Ｐ＜０．０１），未转移者６５％（２６／４０）较转移者３０％（６／２０）（Ｐ＜０．０１），临床Ⅳ期４０％（６／１５）较Ⅰ期８２％（９／１１）、Ⅱ期７１％（３０／４２）（Ｐ＜０．０５）皆具有显著性差异。结论Ｒｂ缺失与胃癌发生、浸润与转移及临床分期有关，从分子水平证实中度、重度不典型增生是癌前病变，检测Ｒｂ蛋白有助于胃癌的诊断、生物学行为及预后的评估
Objective To observe the expression of Rb protein in gastric cancer and its relationship with invasion, metastasis and clinical stage. Methods The expression of Rb protein in normal gastric mucosa, atypical hyperplasia and gastric cancer was detected by streptavidin-peroxidase linkage method. Results The expression rate of Rb protein was 100% (10/10) in normal gastric mucosa, which was significantly higher than that of atypical hyperplasia (80%, 48/60) (P<0.05) and gastric cancer, 60% (72/120) (P<0). .01); Atypical hyperplasia and gastric cancer were significantly different (P <0.01), but moderate 80% (16/20), severe 75% (12/20) but no significant; well-differentiated adenocarcinoma 68 % (23/34), poorly differentiated adenocarcinoma 58% (29/50), undifferentiated carcinoma 64% (9/14), mucinous carcinoma 50% (6/12), signet-ring cell carcinoma 50% (5/10 There was no significant difference among the various types; 75% (30/48) of the infiltrated superficial muscles were 50% (36/72) more infiltrating the deeper muscular layers and full layers (P<0.01). % (26/40) was more than 30% (6/20) of metastases (P<0.01), clinical stage IV 40% (6/15) was more than stage I 82% (9/11), stage II 71% ( 30/4 ) (P <0.05) are significantly different. Conclusion Rb deletion is related to the occurrence, invasion and metastasis of gastric carcinoma and clinical stage. It is confirmed from the molecular level that moderate to severe dysplasia is a precancerous lesion. Detection of Rb protein contributes to the diagnosis, biological behavior and prognosis of gastric cancer.