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[目的]了解梨果实接种梨轮纹病原菌后的防御机制和生防菌的酶活作用机理。[方法]采用不同处理在梨果实上接种梨轮纹病原菌和喷施生防菌,测定其对梨果实抗氧化酶体系的影响。[结果]丙二醛(MDA):生防菌处理对MDA含量变化影响不大,轮纹菌处理MDA含量48 h达到高峰值,为10.22 nmol/g,是对照的1.86倍,轮纹菌+生防菌处理MDA含量24 h达到高峰值,为8.92 nmol/g,是对照的1.62倍;超氧物歧化酶(SOD):生防菌处理的SOD酶活值48 h达到高峰,为126.69 U/[g(FW)·min],是对照的1.54倍,轮纹菌与轮纹菌+生防菌处理均在24 h出现活性高峰,酶活值分别为122.10和135.32 U/[g(FW)·min],是对照的1.48和1.65倍;过氧化物酶(POD):生防菌、轮纹菌、轮纹菌+生防菌处理的POD酶活均在24 h达到高峰值,分别为385.34、342.50、290.00 U/[g(FW)·min],为对照的1.83、1.62、1.38倍;过氧化氢酶(CAT):生防菌、轮纹菌、轮纹菌+生防菌处理的CAT酶活在6h时均达到高峰值,分别为133.33、114.17和113.35 U/[g(FW)·min],为对照的1.33、1.14和1.13倍;多酚氧化酶(PPO):生防菌处理和对照差异不明显,轮纹菌处理酶活高峰出现在12h,为81.86 U/[g(FW)·min],为对照的1.76倍,轮纹菌+生防菌处理酶活高峰出现在24 h,为70.00 U/[g(FW)·min],为对照的1.50倍。[结论]轮纹菌和轮纹菌+生防菌对MDA含量影响较大;轮纹菌及生防菌都能激发SOD酶活性的升高;接种轮纹菌及喷施生防菌都能激发POD酶活性的升高;轮纹菌及生防菌都能激发CAT酶活的升高;单独施用生防菌效果不明显,轮纹菌更能激发PPO酶活性的升高。
[Objective] To understand the defense mechanism of pear fruit after inoculation with Rhizoctonia solani and the biodegradation mechanism of enzyme activity. [Method] Different pathogenic bacteria were inoculated on pear fruit and sprayed on the pest control and antimicrobial effect. The effect on the antioxidant enzyme system of pear fruit was tested. [Result] Malondialdehyde (MDA): biocontrol had little effect on the content of MDA. The contents of MDA in ring rot reached the peak value of 10.22 nmol / g at 48 h, which was 1.86 times of the control, The content of MDA in biocontrol microbes reached a peak value of 8.92 nmol / g at 24 h, which was 1.62 times of that of the control. The activity of superoxide dismutase (SOD) / [g (FW) · min], which is 1.54 times of that of the control. The peak of activity was observed at 24 h for both rotifer and rotifer / biocontrol bacteria. The activity values were 122.10 and 135.32 U / g ) · Min], which were 1.48 and 1.65 times of that of the control respectively. POD activity of POD: biocontrol bacteria, rotifer, rotifer + biocontrol bacteria all reached their peak at 24 h, (385.34,342.50,290.00 U / [g (FW) · min], which were 1.83,1.62,1.38 times of that of the control. Catalase (CAT): biocontrol bacteria, rotifer, rotifer + biocontrol bacteria The CAT activity reached the peak value at 6h, which was 133.33, 114.17 and 113.35 U / [g (FW) · min], which were 1.33, 1.14 and 1.13 times of the control, respectively. Polyphenol oxidase (PPO) There was no obvious difference between the antimicrobial treatment and the control, and the peak of enzyme activity of the rotifers was 12.86 U / [g (FW) · m in], which was 1.76 times of the control. The peak of enzyme activity of rotifer + biocontrol bacteria appeared in 24 h, which was 70.00 U / [g (FW) · min], which was 1.50 times of the control. [Conclusion] The content of MDA in rotifer and rotifer + biocontrol bacteria was greatly affected. Both rotten fungus and biocontrol bacteria could stimulate the increase of SOD activity. And the activity of POD was increased. The growth of CAT activity was stimulated by rotifer and biocontrol bacteria. The biocontrol effect was not obvious and the activity of PPO enzyme was enhanced by rotifer.