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目的 探讨HCT116细胞和HCT116球细胞的成瘤能力及其蓬乱蛋白3(dishevelled 3,DVL3)的表达.方法 采用无血清培养法培养人结直肠癌HCT116细胞,以获得HCT116球细胞,并通过克隆形成实验(3个复孔)和裸鼠皮下成瘤实验(20只裸鼠),分别在体内、外观察HCT116细胞及HCT116球细胞的集落形成能力和成瘤能力,再采用Western blotting法检测DVL3在2种细胞中的表达情况.结果 ①集落形成情况:HCT116细胞组的克隆形成率均值为3.78％,HCT116球细胞组为28.67％,HCT116球细胞组的克隆形成率较高(t=21.16,P＜0.05).②裸鼠皮下成瘤能力:HCT116细胞组形成肿瘤1 1只,肿瘤形成率为55.0％;HCT116球细胞组形成肿瘤18只,肿瘤形成率为90.0％,高于HCT116细胞组(P=0.039);HCT116细胞组裸鼠的肿瘤体积为(92±31) mm3,HCT116球细胞组为(298±85)mm3,HCT116球细胞组裸鼠的肿瘤体积较大(t=9.27,P＜0.05).③DVL3的表达:HCT116细胞中DVL3的表达水平为0.12±0.05,HCT116球细胞为0.35±0.10,HCT116球细胞中DVL3的表达水平较高(t=4.31,P＜0.05).结论 HCT116球细胞较HCT116细胞具有更强的促集落形成能力和致瘤能力,且DVL3的高表达可能与HCT116球细胞较强的致瘤能力有关.“,”Objective To explore the tumorigenicity and expressions of dishevelled 3 (DVL3) in HCT116 cells and HCT116 spherical cells.Methods Human colorectal tumor HCT116 cells were cultured in the serum-free culture medium for HCT11 6 spherical cells.Through the subcutaneous tumor experiment in nude mice and clone formation assay,we observed the tumor growth and colony formation ability of the two kinds of cells in vivo and in vitro.The Western blotting experiment was utilized to detect the expressions of DVL3 in these two kinds of cells.Results ① Colony formation:the mean value of colony formation rate in the HCT 116 cells group was 3.78％,and the mean value of fcolony formation rate in the HCT116 spherical cells group was 28.67％,which was higher in the HCT116 spherical cells group (t=21.16,P＜0.05).② Tumorigenicity in nude mice:11 nude mice with tumor formation were observed in the HCT116 cells group,and the tumor formation rate was 55.0％;18 nude mice with tumor formation were observed in the HCT116 spherical cells group,and the tumor formation rate was 90.0％,the tumor formation rate of the HCT 116 spherical cells group was higher (P=0.039).The tumor volume of the HCT116 cells group was (92±31) mm3,and the tumor volume of HCT116 spherical cells group was (298±85) mm3,the tumor volume of the HCT116 spherical cells group was larger (t=9.27,P＜0.05).③ The expression of DVL3:the expression level of DVL3 in HCT116 cells was 0.12±0.05,and expression level of DVL3 in HCT116 spherical cells was 0.35±0.10,the expression level of DVL3 in HCT116 spherical cells was higher (t=4.31,P＜0.05).Conclusions The HCT116 spherical cells have stronger colonization and tumorigenicity than the HCT116 cells.It has been speculatd that the high expression of DVL3 may be closely related with the stronger tumorigenicity.