目的　解决南方红豆杉生长缓慢的问题。方法　考察红豆杉细胞悬浮培养过程中磷的消耗 ,并研究了碳、氮、磷对细胞生长及细胞活性的影响。结果　碳源在悬浮培养中期已经消耗殆尽 ,后期碳源的缺乏限制了细胞的生长。结论　补加碳源组与对照组相比 ,细胞生长率和细胞密度明显提高 ,其中细胞生长率约提高了 83%。 Objective To solve the problem of slow growth of Taxus chinensis. Methods Phosphorus consumption during suspension culture of Taxus chinensis was investigated. The effects of carbon, nitrogen, and phosphorus on cell growth and cell viability were studied. Results Carbon sources have been depleted in the middle of suspension culture. The lack of carbon sources at the later period limits the growth of cells. Conclusion Compared with the control group, supplemental carbon source group significantly increased cell growth rate and cell density, and the cell growth rate increased by about 83%.