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以双醛葡聚糖-组氨酸偶连物(PAD-His)为功能大单体、过渡态类似物p-硝基苯磷酸酯(NPP)为模板分子、Co2+为中心离子,采用油包水反相乳液法首次制得具有酯酶活性的温敏型分子印迹微凝胶(MIGs).催化水解实验表明,MIGs催化活性受模板分子用量的影响,并可通过温度进行有效调控.MIGs催化p-硝基乙酸苯酯(NPA)水解反应行为可用Michaelis-Menten方程进行描述,其最大催化水解反应速率和Michaelis-Menten常数分别为2.04×10-8mol/h和2.2×10-5mol/L,且具有较好的催化选择性.
His experiment was carried out using PAD-His as functional macromonomer, transition state analogue p-nitrophenylphosphate (NPP) as template molecule and Co2 + as center ion. Oil-in-water Water-in-phase emulsion method was used to prepare temperature-sensitive molecularly imprinted microgels (MIGs) with esterase activity for the first time.Catalytic hydrolysis experiments showed that the catalytic activity of MIGs was affected by the amount of template molecule and could be effectively regulated by temperature. The hydrolysis reaction of p-nitroacetate (NPA) can be described by Michaelis-Menten equation. The maximum catalytic hydrolysis rate and Michaelis-Menten constant are 2.04 × 10-8 mol / h and 2.2 × 10-5 mol / And has better catalytic selectivity.