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目的 :观察平颤汤对帕金森病模型动物的行为影响和脑内多巴胺含量及其代谢的影响 ,以探讨其治疗帕金森病的机理。方法 :应用 6 - OHDA立体定向损毁大鼠单侧黑质DA神经元诱发动物旋转 ,观察平颤汤能否改变其旋转行为并测定纹状体多巴胺及其代谢产物的变化 ;应用氧化震颤素 ,使小鼠产生震颤行为 ,观察平颤汤能否拮抗其震颤行为 ;应用槟榔碱诱发小鼠震颤行为 ,观察平颤汤组成中白芍、钩藤两组份有无拮抗作用 ,并与安坦作对照。结果 :平颤汤对 6 - OHDA造模的大鼠旋转行为无影响 ,也不能增加其纹状体多巴胺含量 ;平颤汤 144 g/ kg连续灌胃 3d,有较明显的抗震颤作用 ;拆方试验 ,白芍、钩藤两组份未能拮抗槟榔碱引起的小鼠震颤行为。提示 :平颤汤未有兴奋脑内多巴胺受体功能的作用 ,也不能增加神经递质的含量 ;但有一定的对抗震颤作用 ,而其拆方两组份无此作用
Objective : To observe the behavioral effects of Ping Que Tang on Parkinson’s disease model animals and the influence of dopamine content and metabolism in brain to explore the mechanism of its treatment for Parkinson’s disease. METHODS: Rat unilateral substantia nigra DA neurons were induced to rotate by 6-OHDA stereotaxic rats, and whether the Pinghuo Tang was able to change its rotation behavior and the changes of striatum dopamine and its metabolites were detected; The mice were tremored and observed whether the Pinghuo Tang could antagonize its tremor behavior. The betel-acuite was used to induce the tremor behavior of the mice, and whether the two components of the Pinghuo Tang composition had antagonistic effects were observed. For comparison. Results: Pingchan decoction had no effect on the rotational behavior of rat model of 6-OHDA, nor could it increase the content of dopamine in striatum. Pinghuotang decoction was 144 g / kg continuous gavage for 3 days, and it had obvious anti-tremor effect. In the square test, the two groups of white peony and uncaria failed to antagonize the tremor behavior of mice induced by arecoline. Tip : Pinghuotang does not stimulate the function of dopamine receptors in the brain, nor does it increase the content of neurotransmitters; however, it has a certain degree of anti-tremor effect, and its disassembled two groups have no effect.