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本研究挖掘番茄(Solanum lycopersicum)中NAC转录因子家族成员104个,为研究番茄NAC转录因子在响应生物及非生物胁迫中的调控作用提供理论基础,也为番茄NAC转录因子的全基因组分析提供了依据。通过SOL数据库下载番茄NAC、NAM基因及蛋白序列,根据NAC保守域序列号PF01849、PF02365,利用HMMER 3.0软件鉴定番茄NAC基因。再利用保守结构域预测软件Pfam和SMART对候选序列进行筛选鉴定。使用DNAMAN5.0、Web Logo、MEGA5.2、GSDS2.0、Map Inspect、Ex Pasy和Swiss-Model等软件对获选番茄NAC转录因子进行生物信息学分析。通过RT-PCR技术检测番茄NAC转录因子在不同组织中的表达情况。本实验通过生物信息学方法对番茄中NAC基因家族成员进行了预测及分析。预测及分析结果显示,番茄NAC基因家族包含104个蛋白质,通过分组鉴定和进化树分析将番茄NAC基因家族分为12个亚族,番茄特有的TNAC亚族成员最多为28个。本实验对挖掘到的104个NAC转录因子的分子量、等电点、保守结构域及蛋白质二、三级结构进行了预测及分析。本实验选取12个NAC转录因子家族成员进行RT-PCR技术检测,获得其在根、茎、叶、花、果中的表达情况,半定量结果显示,7个Sl NAC基因在根、茎、叶、花、果(红熟)中均有表达,并呈现出多种表达模式。番茄NAC转录因子家族在结构上高度保守,并参与番茄生长发育、生物及非生物胁迫的调控过程。
In this study, 104 NAC transcription factor family members from Solanum lycopersicum were excised to provide a theoretical basis for studying the regulatory role of NAC transcription factors in response to biotic and abiotic stresses and to provide a genome-wide analysis of tomato NAC transcription factors in accordance with. The tomato NAC and NAM genes and protein sequences were downloaded from the SOL database. The tomato NAC genes were identified by HMMER 3.0 software according to the NAC conserved domain sequences PF01849 and PF02365. The conserved domain prediction software Pfam and SMART were used to screen the candidate sequences. Bioinformatic analysis of the NAC transcription factor of tomato was carried out by using software such as DNAMAN5.0, Web Logo, MEGA5.2, GSDS2.0, Map Inspect, Ex Pasy and Swiss-Model. The expression of tomato NAC transcription factor in different tissues was detected by RT-PCR. In this study, bioinformatics methods were used to predict and analyze NAC gene family members in tomato. The results of prediction and analysis showed that the NAC gene family of tomato contained 104 proteins, and the NAC gene family of tomato was divided into 12 subgroups by cluster analysis and phylogenetic tree analysis. The members of the tomato-specific TNAC subfamily had a maximum of 28 members. In this study, we predicted and analyzed the molecular weight, isoelectric point, conserved domains and the secondary and tertiary structures of 104 NAC transcription factors. In this experiment, 12 NAC transcription factor family members were detected by RT-PCR, and their expression in roots, stems, leaves, flowers and fruits was obtained. The semi-quantitative results showed that there were seven NAC genes in roots, stems and leaves , Flowers, fruit (red ripe) are expressed, and showed a variety of expression patterns. The NAC transcription factor family of tomato is structurally highly conserved and is involved in the regulation of tomato growth and development, as well as biotic and abiotic stress.