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目的:探讨不同电化学治疗剂量对肿瘤细胞生长周期的影响、细胞周期变化特点及对宫颈癌细胞系CDK和cyclin表达的影响。方法:用体外实验方法,用不同电化学剂量处理宫颈癌细胞系后分别用流式细胞仪和RT-PCR测定细胞周期分布及细胞CDK和cyclin mRNA的表达。结果:(1)不同电化学治疗剂量组合作用后S期细胞所占比例均低于空白组,差异有统计学意义(P<0.01),但G1细胞所占比例均高于空白组,差异有统计学意义(P<0.01)。G2期细胞所占比例与空白对照的差异无统计学意义(P>0.05);(2)(5V,10C)、(10V,10C)处理组cyclin D1表达阴性;(5V,5C)、(10V,5C)处理组cyclinD1表达阳性。各电化学剂量处理组CDK4、CDK1、cyclin B1表达均阳性;(3)不同电化学治疗剂量组合作用后各基因表达与G2期比例无明显相关,但cyclinD1基因表达与G1期呈负相关,与S期呈正相关。CDK1基因表达与G1期呈正相关,与S期呈负相关。结论:电化学治疗能通过调节肿瘤细胞CDK和cyclin基因的表达影响其细胞周期各时相的分布。
OBJECTIVE: To investigate the effects of different doses of chemotherapy on the growth of tumor cells, the characteristics of cell cycle and the expression of CDK and cyclin in cervical cancer cell lines. Methods: The cell cycle distribution and the expression of CDK and cyclin mRNA were determined by flow cytometry and RT-PCR respectively in vitro with different electrochemical doses of cervical cancer cell lines. Results: (1) The percentage of S phase cells in different electrochemical treatment groups was lower than that in blank group (P <0.01), but the proportion of G1 cells was higher than that in blank group Statistical significance (P <0.01). (2) The expression of cyclin D1 was negative at (5 V, 5 C), (10 V, 10 C) and (10 V, 10 C), and the percentage of G2 phase cells was not significantly different from that of the blank control group , 5C) treatment group cyclinD1 expression was positive. The expressions of CDK4, CDK1 and cyclin B1 were all positive in each electrochemical dose group. (3) There was no significant correlation between the expression of CDK4, CDK1 and cyclin B1 in different electrochemical treatment groups, but the expression of cyclinD1 was negatively correlated with the G1 phase S phase was positively correlated. CDK1 gene expression was positively correlated with G1 phase and negatively correlated with S phase. Conclusion: Electrochemical therapy can affect the distribution of CDK and cyclin genes in tumor cells at different phases of cell cycle.