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目的探讨地西他滨(decitabine,DCA)和丙戊酸钠(valproic acid,VPA)联用对白血病细胞株HL-60的促分化影响。方法设立分组如下:对照组,DCAA组(1.0 mol.L 1),DCA B组(4.0 mol.L 1),VPA组(2.0 mmol.L 1),联合用药A组(DCA1.0 mol.L 1+VPA 2.0 mmol.L 1),联合用药B组(DCA 4.0 mol.L 1+VPA 2.0 mmol.L 1),作用48 h。流式细胞术检测CD34、CD117 MFI以及CD11b、CD14表达率。结果联合用药A、B组的CD117和CD34 MFI显著低于其各自的单药组(P<0.01);联合用药A组的CD11b和CD14表达率和联合用药B组CD11b表达率显著低于其各自的单药组(P<0.01)。结论 VPA与DCA联用对HL 60细胞具促分化作用。
Objective To investigate the effect of decitabine (DCA) and valproic acid (VPA) on the differentiation of leukemia cell line HL-60. Methods The following groups were established: DCAA group (1.0 mol.L 1), DCA B group (4.0 mol.L 1), VPA group (2.0 mmol.L 1), combination group A (DCA 1.0 mol.L 1 + VPA 2.0 mmol.L 1), combined group B (DCA 4.0 mol.L 1 + VPA 2.0 mmol.L 1) for 48 h. Flow cytometry was used to detect the expression of CD34, CD117 MFI and CD11b, CD14. Results The MFI of CD117 and CD34 in group A and group B were significantly lower than those in group A (P <0.01). The expression of CD11b and CD14 in group A and the expression of CD11b in group B were significantly lower than those in group A Of monotherapy group (P <0.01). Conclusions VPA combined with DCA can promote the differentiation of HL 60 cells.