Objective: To determine if aquaporin-1 could be detected in cultures of human trabecularmeshwork cells.Methods: Using primers specific for aquaporin-1, reverse transcription combined withpolymerase chain reaction (RT-PCR) yielded a product and its size with total RNAprepared from the human trabecular meshwork cells. SDS-PAGE and immunoblottingwere also used in this study to detect the specific water channel.Results: The presence of this product and its size (298 base pairs) are consistent withthat of an aquaporin-1 message in these cells. A band of 28 kD in agreement with themolecular size of aquaporin-1 was showed in a film by immunoblotting.Conclusion: The presence of aquaporin-1 in human trabecular meshwork cells, thepredominant cell-type of the primary outflow region of the human eye, suggests that waterchannels may be involved in the movement of aqueous fluid out of the eye. In addition,the existence of aquaporin-1 on cultures of human trabecular meshwork cells provides anin vitro model to study Objective: To determine if aquaporin-1 could be detected in cultures of human trabecular meshwork cells. Methods: Using primers specific for aquaporin-1, reverse transcription combined with polymerase chain reaction (RT-PCR) yielded a product and its size with total RNAprepared from the Results: The presence of this product and its size (298 base pairs) are consistent with the current of aquaporin-1 message in these cells. A band of 28 kD in agreement with the molecular size of aquaporin-1 was showed in a film by immunoblotting. Conlusion: The presence of aquaporin-1 in human trabecular meshwork cells, the predominant cell-type of the primary outflow region of the human eye, suggests that waterchannels may be involved in the movement of aqueous fluid out of the eye. In addition, the existence of aquaporin-1 on cultures of human trabecular meshwork cells provides anin vi tro model to study