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目的研究花色苷对小鼠腹腔巨噬泡沫细胞胆固醇外流的影响,探讨花色苷抗动脉粥样硬化的分子机制。方法以50μg/ml 氧化型低密度脂蛋白培养小鼠腹腔巨噬细胞24 h 形成巨噬泡沫细胞。用1、10、100μmoL/L 矢车菊定-3-葡萄糖苷(Cy-3-g)和芍药定-3-葡萄糖苷(Pn-3-g)分别培养巨噬泡沫细胞,酶学荧光法检测培养液中胆固醇含量,用定量 PCR 和激光共聚焦显微镜检测三磷酸腺苷结合盒转运体 A1(ABCA1)的表达。结果花色苷 Cy-3-g 和 Pn-3-g 能够促进巨噬泡沫细胞胆固醇外流,这一作用存在时间和剂量-依赖关系。花色苷还能促进巨噬泡沫细胞 ABCA1 mRNA 和蛋白的表达,ABCA1抑制剂4,4’-二异硫氰酸二丙乙烯-2,2’-二磺酸能够阻断花色苷诱导巨噬泡沫细胞胆固醇外流。结论花色苷 Cy-3-g 和 Pn-3-g 通过调控 ABCA1的表达从而促进巨噬泡沫细胞胆固醇外流。
Objective To study the effect of anthocyanins on cholesterol efflux in murine peritoneal macrophage foam cells and to explore the molecular mechanism of anthocyanin against atherosclerosis. Methods Mice peritoneal macrophages were cultured with 50μg / ml oxidized low density lipoprotein for 24 hours to form macrophage foam cells. Macrophage foam cells were cultured with 1, 10, 100μmoL / L Cy-3-g and Pn-3-g, respectively. Enzyme- The content of cholesterol in the culture medium was detected. The expression of ATP-binding cassette transporter A1 (ABCA1) was detected by quantitative PCR and confocal laser scanning microscopy. Results Anthocyanins Cy-3-g and Pn-3-g promoted cholesterol efflux in macrophage foam cells, and the effect of time and dose-dependent relationship existed. Anthocyanins also promote ABCA1 mRNA and protein expression in macrophage foam cells. The ABCA1 inhibitor 4,4’-diisothiocyanate-2,2’-disulfonic acid blocked anthocyanin-induced macrophage foam Cellular cholesterol efflux. Conclusions Anthocyanins Cy-3-g and Pn-3-g can promote the cholesterol efflux in macrophage foam cells by regulating the expression of ABCA1.