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目的:探讨重楼醇提物和水提物对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用。方法:小鼠随机分为9组,分别每天给予不同剂量的药物:重楼醇提物高、中、低剂量组(3.9、2.6、1.3 g/kg)、水提组的高、中、低剂量组(7.2、4.8、2.4g/kg)和联苯双酯(0.15 g/kg),正常组和模型组给予生理盐水。7d后,除正常对照组外各组小鼠腹腔注射0.1%CCl4制备急性肝损伤模型,16h后取血检测血清ALT、AST活性;测定肝组织匀浆中MDA含量和SOD、GSH的活性及观察肝脏病理学变化。结果:醇提组高剂量(3.9g/kg)和水提物高、中、低剂量(7.2、4.8、2.4g/kg)组均能降低CCl4所致急性肝损伤小鼠血清ALT、AST活性(P<0.01,P<0.05),降低肝脏MDA的含量、增强SOD和GSH活力(P<0.01,P<0.05),并能明显改善肝组织的病理学损伤。结论:重楼水提物对CCl4所致小鼠急性肝损伤具有较好保护作用。
Objective: To investigate the protective effects of ethanol extracts and water extracts of Cortex Fragrans on acute liver injury induced by carbon tetrachloride (CCl4) in mice. Methods: The mice were divided into 9 groups at random, and were given different doses of drugs daily: high, medium and low dose alcohol extract (3.9,2.6,1.3 g / kg), high, medium and low (7.2,4.8,2.4 g / kg) and bifendate (0.15 g / kg). The normal group and model group were given normal saline. After 7 days, the mice in each group were given intraperitoneal injection of 0.1% CCl4 to prepare acute hepatic injury model. After 16 hours, the serum ALT and AST levels were measured. The content of MDA and the activities of SOD and GSH in liver homogenates were measured and observed Liver pathology changes. RESULTS: The high, middle and low dose (7.2,4.8,2.4 g / kg) groups of alcohol extract group (3.9 g / kg) and water extract group decreased the activity of ALT and AST in mice with acute liver injury induced by CCl4 (P <0.01, P <0.05), decreased the content of MDA in the liver, increased the activity of SOD and GSH (P <0.01, P <0.05), and significantly improved the pathological damage of liver tissue. Conclusion: Extract from Rhizoma Paridis has a good protective effect on acute liver injury induced by CCl4 in mice.