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目的研究焦炉逸散物对焦炉工人外周血淋巴细胞热休克蛋白72(Hsp72)水平的影响及与DNA损伤的关系,探讨Hsp72在细胞损伤保护中的意义。方法选取267名焦炉工人和30名对照,以Western blot法检测外周血淋巴细胞Hsp72的水平,彗星试验检测DNA损伤水平,个体采样采集作业环境空气样本,高效液相色谱法测定环境苯并[a]芘浓度。结果高暴露组工人外周血淋巴细胞Hsp72水平(G±S_G)和Olive尾矩(G±S_G)分别为1.24±0.42和4.49±1.24,明显高于低暴露组(1.01±0.35和2.99±1.10)和对照组(0.85±0.34和2.40±1.00),差异有统计学意义(P<0.05)。以全体研究对象Hsp72中位数为界值,将研究对象分为Hsp72高表达组和低表达组,Hsp72高表达的人数相对比率在对照组、低暴露组、高暴露组分别为36.7%、43.1%和58.3%,呈逐渐增高趋势,差异有统计学意义(P=0.003)。在Hsp72高表达组,Hsp72表达水平随着暴露等级升高而升高,其中高暴露组与对照组的差异有统计学意义(P<0.05),但各组之间Olive尾矩的差异无统计学意义(P>0.05)。在Hsp72低表达组中,各组Hsp72水平的差异无统计学意义(P>0.05),而Olive尾矩随暴露等级升高而升高,其中高暴露组与低暴露组和对照组比较,差异有统计学意义(P<0.01),且明显高于Hsp72高表达组的高暴露组工人。高暴露组Hsp72水平与Olive尾矩呈负相关(r=-0.503,P<0.01)。结论焦炉逸散物可诱导焦炉工人外周血淋巴细胞Hsp72水平升高,Hsp72对细胞DNA损伤具有保护作用。
Objective To investigate the effect of Coke Oven on the level of heat shock protein 72 (Hsp72) in peripheral blood lymphocytes and its relationship with DNA damage, and to explore the significance of Hsp72 in cell injury protection. Methods A total of 267 coke oven workers and 30 controls were selected to detect the level of Hsp72 in peripheral blood lymphocytes by Western blot, DNA damage levels in comet assay, ambient air samples in individual samples and high performance liquid chromatographic determination of benzo [ a] pyrene concentration. Results The levels of G ± S_G and G ± S_G of peripheral blood lymphocytes in high exposure group were 1.24 ± 0.42 and 4.49 ± 1.24 respectively, which were significantly higher than those in low exposure group (1.01 ± 0.35 and 2.99 ± 1.10) And control group (0.85 ± 0.34 and 2.40 ± 1.00), the difference was statistically significant (P <0.05). According to the median of Hsp72 in all subjects, the subjects were divided into Hsp72 overexpression group and low expression group. The relative ratio of Hsp72 overexpression in control group, low exposure group and high exposure group were 36.7%, 43.1 % And 58.3%, respectively, showing a gradual increase trend, the difference was statistically significant (P = 0.003). In Hsp72 overexpression group, the expression level of Hsp72 increased with the increase of exposure level, and the difference between the high exposure group and the control group was statistically significant (P <0.05), but there was no statistical difference Significance (P> 0.05). In Hsp72 low expression group, there was no significant difference in Hsp72 level between groups (P> 0.05), while the Olive tail moment increased with the increase of exposure level, in which the difference between high exposure group and low exposure group and control group (P <0.01), and was significantly higher than those in the high-exposure group with Hsp72 overexpression group. The Hsp72 level in high exposure group was negatively correlated with Olive tail moment (r = -0.503, P <0.01). Conclusion Coke oven fugitives can induce Hsp72 levels in peripheral blood lymphocytes of coke oven workers, and Hsp72 has a protective effect on DNA damage.