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目的开展川续断种质资源的遗传多样性研究,为合理利用川续断种质资源提供理论依据。方法运用SRAP分子标记方法对川黔境内川续断的遗传多样性进行分析。结果 10对引物共检测到124个位点,其中102个位点具有多态性,多态位点百分率(PPL)为82.26%。川续断总的Nei’s基因多样性指数(H)为0.280 0,Shannon’s多态性信息指数(I)为0.435 3;居群水平上川续断的PPL为53.92%,H为0.121 2～0.244 0、I为0.179 6～0.361 1,其中5个高海拔、小生境特征的居群遗传多样性指标较高。居群间的基因分化系数Gst为0.293 0,基因流(Nm)为1.206 4。基于遗传相似度,14个居群可聚为3类。结论川续断居群的遗传多样性水平丰富,遗传变异主要存在居群内,地理位置(海拔)和气候是川续断居群遗传多样性较高的影响因素,而地理隔离(小生境)是造成居群内遗传变异高于居群间的另一因素。
OBJECTIVE: To study the genetic diversity of Chuanxiong Chuanxiong germplasm resources and provide theoretical basis for rational utilization of Chuanxiong Chuanxiong germplasm resources. Methods SRAP molecular markers were used to analyze the genetic diversity of Chuanqian in Sichuan and Guizhou. Results Ten pairs of primers were detected in 124 loci, of which 102 loci were polymorphic and the percentage of polymorphic loci (PPL) was 82.26%. The total Nei’s gene diversity index (H) was 0.280 0, and Shannon’s polymorphism information index (I) was 0.435 3. The population level of PPD was 53.92%, H was 0.121 2 ~ 0.244 0, I ranged from 0.179 6 to 0.361 1. Among them, the genetic diversity of five high altitude and niche habitats was high. The coefficient of genetic differentiation (Gst) between populations was 0.293 0, and the gene flow (Nm) was 1.206 4. Based on genetic similarity, 14 populations can be clustered into 3 categories. CONCLUSION: The genetic diversity of the habitats of Chuan dipsaci is rich in genetic diversity. The genetic variation mainly exists in the population. Geographical location (elevation) and climate are the influential factors of high genetic diversity. However, geographical isolation (niche) Is another factor that causes the genetic variation within the population to be higher than that among the populations.