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目的观察蛛网膜下腔出血(Subarachnoid hemorrhage,SAH)后大鼠脑皮质细胞外液中兴奋性氨基酸(Excitatory Amino Acids,EAA)[主要包括谷氨酸(Glu)和天冬氨酸(Asp)]水平的变化规律,探讨SAH后脑损害的机制。方法将SD大鼠20只随机分为SAH组和对照组,每组各10只,将微透析探针置入大鼠右侧顶叶皮质,在不同的时间段进行定点采样,样本采用高效液相分析方法进行检测。结果SAH后0hGlu和Asp水平均明显高于对照组(P<0.001,P<0.01),而后Glu虽有波动但均高于基础水平;在SAH后1hAsp水平降至基础水平,25hAsp水平再次显著升高(P<0.01),而后呈升高趋势。结论SAH后大鼠脑皮质细胞外液中Glu和Asp在不同的时限有不同程度的升高,提示两者在SAH后的缺血性脑损伤中均起一定作用,但早期的神经细胞损伤主要与Glu有关,而Asp主要在中后期发挥作用。
Objective To observe the effects of Excitatory Amino Acids (EAA) [mainly including Glu and Asp] in rat cerebral cortex after subarachnoid hemorrhage (SAH) Level changes in order to explore the mechanism of brain damage after SAH. Methods Twenty SD Sprague-Dawley rats were randomly divided into SAH group and control group, with 10 rats in each group. The microdialysis probe was placed into the right parietal cortex in rats and fixed-point sampling was performed at different time points. Phase analysis method for testing. Results The level of Glu and Asp at 0h after SAH was significantly higher than that of the control group (P <0.001, P <0.01), but Glu was fluctuant but higher than the basal level after SAH. Asp level dropped to basal level at 1h after SAH, High (P <0.01), and then increased. Conclusions The levels of Glu and Asp in extracellular fluid of rat cerebral cortex after SAH increase at different time points, suggesting that both of them may play an important role in ischemic brain injury after SAH. However, the early damage of neurons Is related to Glu, while Asp mainly plays a role in the middle and late stages.