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实验研究了不同投喂方式下,牙鲆口服A3α-PG(A3α肽聚糖)对其血清及组织中超氧化物歧化酶(Superoxidedismutase,SOD)、酸性磷酸酶(Acidphosphatase,ACP)及碱性磷酸酶(Alkalinephos-phatase,AKP)活力的影响。结果表明,不同投喂方式对各组织(肝脏、肾脏、肌肉、粘液)中的SOD、ACP和AKP的诱导能力各异。各实验组与对照组相比,不同投喂方式下对SOD的影响,在肝脏、肾脏、肌肉中未见显著差异(P>0.05);而粘液中的SOD活力在“连续”组“、1+1”组和“4+3”组上升较快,其值约为对照组的两倍,差异显著(P<0.05)。ACP活性在肝脏中以“连续”组和“1+1”组诱导力最强,其与对照组“、4+3”及“7+7”组相比差异显著(P<0.05);而肾脏和肌肉中ACP活力在实验中未表现出差异显著性(P>0.05)。AKP活力在肌肉与粘液中各饲喂频度下未见明显差异;而仅在肝脏与肾脏中表现出对AKP的增强作用,尤以“连续”组和“1+1”组最为明显(P<0.05)。就不同口服方式下,A3α-PG对牙鲆血清中酶活的影响,其诱导能力是有限的,SOD和AKP在血清中的水平均未见明显提高,各实验组与对照组相比基本持平。ACP活性也只在“1+1”方式下有较大提高,并显现出与对照及其它各组之间的差异显著性(P<0.05)。此次实验结果显示,A3α-PG作为一种天然的免疫多糖,能对牙鲆体内氧化酶与水解酶系统产生积极的调理作用,但其作用效果并非以不间断的连续刺激好,而是存在一个较为合适的刺激频度及投喂方式的选择。从实验的总体结果来看,对以上3种酶的调节力以“1+1”和“4+3”方式为好。
The effects of oral administration of A3α-PG (A3α-peptidoglycan) on serum and tissue superoxide dismutase (SOD), acid phosphatase (ACP) and alkaline phosphatase (Alkalinephos-phatase, AKP) activity. The results showed that the induction of SOD, ACP and AKP in different tissues (liver, kidney, muscle and mucus) varied with different feeding modes. There was no significant difference in the SOD, SOD, MDA in mucus between the experimental group and the control group (P> 0.05) +1 “group and” 4 + 3 “group increased rapidly, the value of about twice as much as the control group, the difference was significant (P <0.05). The ACP activity in the liver was the strongest in the ”continuous“ and ”1 + 1“ groups, which was significantly different from the control group ”4 + 3“ and ”7 + 7“ ACP activity in kidney and muscle did not show significant difference in the experiment (P> 0.05). There was no significant difference in the frequency of AKP activity between muscle and mucus, but only in the liver and kidney, which showed the most obvious effect on AKP, especially in ”continuous“ group and ”1 + 1“ group <0.05). The effect of A3α-PG on the activity of Japanese flounder in serum was limited under different oral administration. The levels of SOD and AKP in serum were not significantly increased, and the levels of A3α-PG were almost the same in the control group . ACP activity increased only in the ”1 + 1“ mode, and showed significant difference (P <0.05) compared with the control group and other groups. The experimental results show that A3α-PG as a natural immune polysaccharides can produce a positive conditioning effect on the oxidase and hydrolase system in flounder, but its effect is not good continuous continuous stimulation, but exist A more appropriate frequency of stimulation and feeding method of choice. The overall results from the experiment point of view, the regulation of the above three enzymes to ”1 +1“ and ”4 +3" mode is better.