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目的:建立符合中医理论的实验性肾虚质牙周炎动物模型,为研究牙周炎的中西医结合治疗奠定基础。方法:选用4月龄青年SD大鼠40只,12月龄老龄大鼠40只,随机分为4组:①青年对照组(YN,20只);②青年牙周病组(YP,20只);③老年对照组(ON,20只);④老年牙周病组(OP,20只)。YN组、ON组氯胺酮(100mg/kg)腹腔注射麻醉,常规喂养鼠料和水。YP组、OP组麻醉同对照组,麻醉后将0.2mm正畸钢丝结扎于牙颈部,然后用GC粘结固定,从实验当天开始饲以高糖饮食。结果:术后8周组织病理学检查显示YN组、ON组牙周组织无病理性改变。YP组牙周袋形成,破骨细胞增多,牙槽嵴顶吸收。OP组牙周袋形成,牙槽嵴顶及牙槽骨破坏吸收,牙槽骨吸收近根尖1/3。结论:老年大鼠较青年大鼠易感牙周炎,模拟了临床肾虚质牙周炎自然发生过程,为中西医结合治疗肾虚质牙周炎奠定了基础。
OBJECTIVE: To establish an animal model of experimental periodontitis with kidney deficiency and in line with the theory of traditional Chinese medicine (TCM), which lays the foundation for the study of traditional Chinese medicine and western medicine for periodontitis. Methods: Forty young Sprague-Dawley rats aged 4 months and 40 aged 12-month-old rats were randomly divided into four groups: ① young control group (YN, 20); ② young periodontal disease group (YP, 20 ); The elderly control group (ON, 20); ④The elderly periodontal disease group (OP, 20). YN group, ON group ketamine (100mg / kg) intraperitoneal injection anesthesia, conventional feeding rats and water. YP group and OP group were anesthetized with the control group. After anesthesia, 0.2 mm orthodontic wire was ligatured in the dental neck and then fixed by GC. From the experimental day, high-sugar diet was fed. Results: Histopathological examination at 8 weeks after operation showed no changes in periodontal tissues in YN group and ON group. YP group periodontal pocket formation, osteoclasts, alveolar crest absorption. OP group periodontal pocket formation, alveolar crest and alveolar bone damage absorption, alveolar bone absorption near the apical 1/3. Conclusion: The aged rats are more susceptible to periodontitis in young rats, which simulates the natural process of clinical kidney deficiency periodontitis, which lays the foundation for the treatment of periodontitis with deficiency of kidney by traditional Chinese and western medicine.