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近年来发现雷公藤总甙及其单体T_4及T_(13)有雄性抗生育作用;停药后,生育力可恢复.本实验观察雷公藤总甙及单体的遗传效应,以估价其抗生育作用的安全性.已知精子头部形态由多种基因控制,诱变剂作用于生精细胞,可损伤遗传基因,从而引起精子头部形态异常,造成后代先天性畸形或产生潜在遗传危害.目前,监测精子头部形态已作为检验药物生殖遗传效应的重要指标.本实验采用银染法,使精子头部形态清晰可见,从而增加了对异常精子头部的检出率.将雄性大鼠分成5组:①雷公藤总甙大剂量组
In recent years, it was found that Tripterygium glycosides glycosides and their monomers T_4 and T_ (13) have male antifertility effect; after stopping, fertility can be restored.This experiment observed the genetic effects of Tripterygium glycosides glycosides and monomers in order to evaluate the anti Fertility safety.Some known sperm head morphology controlled by a variety of genes, mutagens acting on spermatogenic cells, can damage the genetic, causing abnormal sperm head morphology, resulting in descendants of congenital malformations or potential genetic hazards At present, the monitoring of sperm head morphology has been used as an important index to test the reproductive genetic effects of drugs.In this experiment, the silver staining method was used to make the sperm head morphology clearly visible, thus increasing the detection rate of abnormal sperm head.Male Rats were divided into 5 groups: ① Tripterygium glycosides large dose group