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目的 探索大动物低剂量全身照射的适宜剂量 ,以便评价低剂量全身照射应用于临床的可能性。方法 食蟹猴 32只随机分为 4组 ,用γ射线 (吸收剂量率为 0 175mGy min)分别照射0、30、5 0和 75mGy。分别在环磷酰胺处理前、后 ,亦即照射前和照射后 1、2、8、33和 5 0周检测淋巴细胞亚群、免疫球蛋白及自然肿瘤红细胞花环试验 (NTERT)。结果 结果显示白细胞释放细胞因子尤其是CD1 6 参与免疫调控 ,出现时间 剂量的反应 ;体液免疫中以IgA升高为特征 ,照射后第 8周 5 0和75mGy 2个剂量组与对照组相比差异有显著性 (P <0 0 5 ) ;NTERT结果显示 ,在照射后第 2周 ,第 1、2、3组分别与第 4组相比差异有显著性 (P <0 0 5 ) ,但第 1组分别与第 2、3组相比差异无显著性。结论 γ射线单次低剂量全身照射对免疫功能低下模型的食蟹猴红、白细胞免疫功能有增强其免疫功能的作用。
Objective To explore the appropriate dose of low-dose whole body irradiation in large animals in order to evaluate the possibility of using low-dose whole-body irradiation in clinical practice. Methods 32 cynomolgus monkeys were randomly divided into 4 groups and irradiated with 0, 30, 50 and 75 mGy respectively by γ-ray (absorbed dose rate of 0 175 mGy min). Lymphocyte subsets, immunoglobulins and natural tumor erythrocyte rosette test (NTERT) were performed before and after cyclophosphamide treatment, ie, before irradiation and at 1, 2, 8, 33 and 50 weeks after irradiation. The results showed that leukocyte release of cytokines, especially CD16 participate immune regulation, the emergence of time dose response; humoral immunity characterized by elevated IgA at 8 weeks after irradiation 50 and 75mGy 2 dose groups compared with the control group (P <0.05). The results of NTERT showed that there was a significant difference between group 1 and group 2 at 4 weeks after irradiation (P <0 05) There was no significant difference between group 1 and group 2 and group 3 respectively. Conclusion A single low-dose whole-body irradiation with γ-rays can enhance the immune function of cynomolg monkey red and white blood cells in immunocompromised mice.