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翡翠宝石(Philodendron spp.)为天南星科蔓绿绒属多年生常绿草本植物,观赏价值高。常规繁殖采用分株法,但速度缓慢,一年只有3～4倍。而通过组培快繁,可以实现周年生产,获得大量的商品苗,有很高的市场价值。培养条件以 MS 为基本培养基(1)初代培养基 MS+6-BA3.0+NAA0.1。(2)继代培养基 MS+6-BA2.0+NAA0.3+AD1.0。(3)生根培养基1/2MS+NAA0.3+IBA0.2。以上培养基均加6克/升国产纯化琼脂粉。培养基(1)、(2)加30克/升绵白糖,(3)加15克/升绵白糖。pH 值5.8;培养温度25±2℃,光照12～14小时/天;光照强度1500～2000Lx。生长与分化情况外植体的灭菌取健壮植株,洗净根部基质,将植株浸于0.1%
Philodendron spp. Is a perennial evergreen herb of Araceae, with high ornamental value. Routine breeding using ramets, but slow, only 3 to 4 times a year. By tissue culture fast propagation, can achieve annual production, access to a large number of commodity seedlings, have a high market value. Culture conditions MS-based medium (1) primary culture medium MS + 6-BA3.0 + NAA0.1. (2) subculture medium MS + 6-BA2.0 + NAA0.3 + AD1.0. (3) rooting medium 1 / 2MS + NAA0.3 + IBA0.2. Above medium plus 6 g / L domestic purified agar powder. Medium (1), (2) 30 g / l soft white sugar, (3) 15 g / l soft white sugar. pH 5.8; culture temperature 25 ± 2 ℃, light 12 ~ 14 hours / day; light intensity 1500 ~ 2000Lx. Growth and differentiation of the explants to take healthy plants sterilization, wash the roots of the substrate, the plant immersed in 0.1%