目的探讨造血干细胞移植后免疫介导的自身免疫性溶血性贫血患者血清血检测方法和处理措施,选择合适的血液品种,最大程度的保障患者输血安全。方法采用盐水介质试管法,聚凝胺法进行抗体筛选,用聚凝胺法鉴定类抗体,采用盐水介质试管法,抗球蛋白法,聚凝胺法进行交叉配血实验。结果患者血清中存在类抗-Ce自身抗体。结论任何类型的同种异体造血干细胞移植后都有可能发展成自身免疫性溶血性贫血,所产生的类同种特异性自身抗体可以针对患者自身红细胞上的抗原。 Objective To investigate the serum test methods and treatment measures for immune mediated hemolytic anemia after hematopoietic stem cell transplantation and to select the appropriate blood breed to ensure the maximum blood transfusion safety. Methods Saline medium test tube method, polybrene method for antibody screening, polybrene method for the identification of antibodies, the use of saline medium test tube, antiglobulin method, polybrene method cross matching blood experiments. Results There were anti-Ce autoantibodies in the patient’s serum. Conclusion All types of allogeneic hematopoietic stem cell transplantation are likely to develop into autoimmune hemolytic anemia, the resulting allospecific autoantibodies against the patient’s own antigens on red blood cells.