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[目的]探讨大气细颗粒物(PM2.5)标准品的水溶性及酸溶性成分对冠状动脉粥样硬化大鼠的致炎作用。[方法]48只Wistar大鼠随机分为对照组和冠状动脉粥样硬化模型组(模型组),每组各24只。对照组饲喂正常饲料,模型组饲喂高胆固醇饲料,12周后冠脉病理切片示模型成功建立。提取PM2.5的水溶性成分(WSC)及酸溶性成分(ASC)。将对照组和模型组再各自随机分为3组,分别为正常对照组、WSC对照组、ASC对照组;以及模型对照组、WSC模型组、ASC模型组,每组8只。WSC对照组和WSC模型组(均称WSC组)尾静脉注射WSC(40mg/kg)染毒,ASC对照组和ASC模型组(均称ASC组)尾静脉注射ASC(40mg/kg)染毒,而正常对照组和模型对照组(均称空白组)则以尾静脉注射生理盐水。染毒24h后处死大鼠,测定血清中的白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的含量及心肌中的核转录因子-κB(NF-κB)活性。[结果]WSC组血清TNF-α水平为(2.83±0.97)ng/mL,高于空白组(2.53±0.76)ng/mL;心肌NF-κB活性为(14.56±10.58)%,高于空白组(7.33±3.97)%。ASC组心肌NF-κB活性为(18.80±17.04)﹪,也高于空白组。冠状动脉粥样硬化模型在升高IL-6、激活心肌NF-κB方面,与WSC、ASC分别存在协同作用。[结论]WSC具有升高TNF-α水平、激活心肌NF-κB活性的作用;ASC也具有激活心肌NF-κB活性的作用。冠状动脉粥样硬化模型还与WSC、ASC染毒具有交互作用,主要表现在升高IL-6水平,激活心肌NF-κB活性的作用上,表明PM2.5水溶性成分及酸溶性成分均可致冠状动脉粥样硬化大鼠的炎症作用。
[Objective] To investigate the water-soluble and acid-soluble components of airborne particulate matter (PM2.5) standard on the inflammatory effect of coronary atherosclerosis rats. [Method] 48 Wistar rats were randomly divided into control group and coronary atherosclerosis model group (model group), 24 rats in each group. The control group was fed with normal diet, the model group was fed with high cholesterol diet, and the pathological model of coronary artery was established successfully after 12 weeks. Water-soluble components (WSC) and acid-soluble components (ASC) of PM2.5 were extracted. The control group and model group were randomly divided into three groups: normal control group, WSC control group and ASC control group; and model control group, WSC model group and ASC model group, with 8 rats in each group. WSC control group and WSC model group (both named WSC group) were injected with tail vein injection of WSC (40mg / kg), ASC control group and ASC model group (both ASC group) The normal control group and model control group (also known as blank group) was injected into the tail vein of saline. The rats were sacrificed 24h after exposure and the contents of IL-6, TNF-α and NF-κB in the myocardium were measured . [Results] The level of TNF-α in WSC group was (2.83 ± 0.97) ng / mL, higher than that in the blank group (2.53 ± 0.76) ng / mL. The activity of NF-κB in myocardium was (14.56 ± 10.58) (7.33 ± 3.97)%. The activity of NF-κB in myocardium in ASC group was (18.80 ± 17.04)%, also higher than that in blank group. Coronary atherosclerosis model has synergistic effect with WSC and ASC in increasing IL-6 and activating NF-κB in myocardium. [Conclusion] WSC can increase the level of TNF-α and activate the activity of NF-κB in myocardium. ASC also has the activity of activating the activity of NF-κB in myocardium. Coronary atherosclerosis model also interacts with WSC and ASC, mainly on increasing the level of IL-6 and activating the activity of NF-κB in myocardium, indicating that the water-soluble and acid-soluble components of PM2.5 Inflammatory effects on coronary atherosclerosis in rats.