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目的研究体外培养的人胚胎膀胱组织来源细胞(humanbladder,HB)在铅暴露不同时间和浓度下的基因表达差异,以探讨铅毒性的作用机制。方法分别使用100、200μg/ml乙酸铅溶液暴露体外培养的HB细胞(浓度为2×103个/ml)8、24h,设置空白对照,提取RNA,进行cDNA基因表达谱芯片实验。芯片扫描结果经归一化处理,设定Ratio值<0.5或≥2为表达有差异基因。结果铅暴露HB细胞导致差异表达的基因共有2406个,其中,在铅暴露不同时间(8、24h)、不同浓度(100、200μg/ml)皆上调的有45个基因,皆下调的有40个基因。结论基因表达因铅暴露的时间和浓度变化而发生改变;差异表达基因的功能与信号传导、细胞代谢密切相关,也与疾病发生发展相关;可以通过分析基因表达谱改变,探悉铅暴露导致机体损伤的原因和致病机制。
Objective To study the gene expression differences of human embryo bladder derived cells (humanbladder) cultured in vitro at different times and concentrations of lead exposure to explore the mechanism of lead toxicity. Methods 100, 200μg / ml lead acetate solution was used to expose HB cells cultured in vitro (2 × 103 cells / ml) for 8 and 24 hours, respectively. Blank control was set and RNA was extracted for cDNA microarray assay. The results of the chip scan were normalized, set Ratio value <0.5 or ≥ 2 to express differentially expressed genes. Results A total of 2406 genes were differentially expressed in lead exposed HB cells. Among them, 45 genes were up-regulated at different concentrations (100 and 200 μg / ml) at different times of lead exposure (8 and 24 hours) gene. Conclusion The gene expression changes due to the time and concentration of lead exposure. The function of differentially expressed genes is closely related to signal transduction and cell metabolism, and is also related to the occurrence and development of the disease. The changes of gene expression profile can be analyzed to find out the lead injury leading to body injury The causes and pathogenesis.