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用中国条锈菌生理小种对欧洲鉴别寄主Heines peko进行抗条锈性遗传机制研究,将为挖掘新的抗条锈基因、培育优良抗条锈性品种奠定基础。用Heinespeko与小麦感病品种铭贤169杂交、自交获F1、F2和F3代群体。对亲本及其后代分别在苗期接种条中30号、条中31号、条中32号和水-4,进行遗传分析。结果表明,Heines peko对条中30号小种的抗性由1对隐性基因控制。对条中31号、水-4的抗性均由1显1隐2对基因互补或抑制作用控制,Heines peko对2个小种的抗性可能由相同基因控制。对条中32号的抗性是由2对隐性基因相互抑制控制。利用分组分析法(BAS)对抗条中30号小种的遗传群体进行分子作图,筛选到1个位于2AS与抗条中30号小种基因连锁的SSR标记Barc212,用Barc212对170个F2代单株分析表明,该基因与Barc212引物扩增位点的遗传距离为10.6cM。Barc212可作为抗条中30号基因的SSR标记。
The genetic mechanism of resistance to stripe rust in Heines peko, a European ecotype host, with Chinese stripe rust races, will lay the foundation for the development of new stripe rust resistance genes and breeding of excellent stripe rust resistance cultivars. Heinespeko was crossed with wheat susceptible cultivar Mingxian169 and self-fertilized F1, F2 and F3 generations. The parents and their offspring were inoculated in the seedlings No. 30, Article 31, Article 32 and water -4, genetic analysis. The results showed that the resistance of Heines peko to race 30 was controlled by one recessive gene. The resistance of No.31 and No.4 in the strip was controlled by the complementary or inhibitory effect of the gene on the loci. The resistance of Heines peko to the two races could be controlled by the same gene. The resistance to number 32 in the bar was controlled by the mutual inhibition of two recessive genes. Molecular mapping was performed by using group analysis (BAS) against the genetic population of race 30 in races. One SSR marker, Barc212, which was linked to race 30 race gene in 2AS and two F2 generations The single plant analysis showed that the genetic distance between the gene and the Barc212 primer amplification site was 10.6 cM. Barc212 can be used as the SSR marker of gene 30 in the anti-tuberculosis.