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目的观察茯苓总三萜(TTP)对小鼠最大电休克模型、戊四唑模型以及大鼠皮层定位注射青霉素诱发癫痫模型的对抗作用。方法采用最大电休克和戊四唑惊厥模型,灌胃小鼠不同剂量茯苓总三萜,以拉莫三嗪(LTG)为对照,观察其抗惊厥作用;建立大鼠皮层定位注射青霉素诱发癫痫模型,以拉莫三嗪为阳性对照,观察两种剂量茯苓总三萜灌胃给药对青霉素诱发癫痫发作和海马区痫性放电的潜伏期、痫波发放频率及痫波最高波幅的影响。结果不同剂量TTP对MES模型均有对抗作用,且量效呈正相关性,但最大效能较拉莫三嗪弱。两种剂量TTP(80 mg/kg,160 mg/kg)均可延长MET发作潜伏期,与对照组比较均有统计学意义(P<0.01),且作用较拉莫三嗪强。两种剂量茯苓总三萜(140 mg/kg,280 mg/kg)和拉莫三嗪(180 mg/kg)均可延长大鼠癫痫发作潜伏期,减轻发作的程度,延长痫性放电的潜伏期(P<0.01),减少痫波发放频率,减小放电最高波幅,与模型组比较,均有统计学意义(P<0.05)。结论茯苓总三萜可明显对抗小鼠最大电休克和戊四唑惊厥,抑制大鼠皮层定位注射青霉素诱发的癫痫发作和痫性放电,产生抗癫痫作用,但抗MES作用较拉莫三嗪弱而抗MET和青霉素惊厥作用较拉莫三嗪强。
Objective To observe the antitumor effect of total triterpenoids (TTP) of Tuckahoe (Tuckahoe) on the model of maximal electroshock in mice, the model of pentylenetetrazole and the model of cortical injection of penicillin-induced epilepsy in rats. Methods The maximum electroshock and pentylenetetrazole seizure model was used. The total triterpenoids of Poria cocos were inoculated in different dosages in mice, and the anticonvulsant effect was observed with lamotrigine (LTG). The model of penicillin-induced epilepsy , Lamotrigine as a positive control to observe the two doses of Poria total triterpenoid administration of penicillin-induced epileptic seizures and epileptic seizures in the hippocampus of the incubation period, the frequency of epileptic waves and the maximum amplitude of the impact of epileptic wave amplitude. Results Different doses of TTP had antagonistic effect on MES model, and the dose-effect was positively correlated, but the maximal efficacy was weaker than that of lamotrigine. Both doses of TTP (80 mg / kg, 160 mg / kg) prolonged the latency of MET onset compared with the control group (P <0.01), and its effect was stronger than that of lamotrigine. Triterpenoids (140 mg / kg, 280 mg / kg) and lamotrigine (180 mg / kg) at both doses both prolonged the seizure latency, reduced the extent of seizure and prolonged the latency of epileptic discharge ( P <0.01), reduce the frequency of epileptic waves and reduce the maximum amplitude of discharge, compared with the model group (P <0.05). Conclusion Total triterpenoids of Poria cocos were able to antagonize the maximal electroshock and pentylenetetrazole seizures in mice and inhibit the epileptic seizures and epileptic discharge induced by injection of penicillin into the cortex of rat cortical, but the effect of anti-MES was weaker than that of lamotrigine The anti-MET and penicillin convulsive effect than lamotrigine.