In this paper predecessors′achievements about the division between subtropical zone and warm temperate zone on the south slope of Funiu Mountain are firstly summarized, and the cause why these viewpoints about the division are different also has been presented. Seven soil profiles at different heights above sea level are dug along the south slope of Funiu Mountain. Many compositions and properties of soil humus have been analyzed in laboratory. A comprehensive study has been made about the division according to the compositions and properties of soil humus with mathematical method. During the analysis process eight indexes have been used, such as altitude, organic carbon, humic acid (HA), fulvic acid (FA), the ratio of humic acid and fulvic acid (HA/FA), two extinction coefficients (E4,E6), and their ratio (E4/E6).The result indicates that the boundary is at about 1000 meters above sea level. In this paper predecessors’achievements about the division between subtropical zone and warm temperate zone on the south slope of Funiu Mountain are one SUMMARY, and the cause why these viewpoints about the division are different also has been presented. Seven soil profiles at different heights above Many comprehensive and properties of soil humus have been analyzed in laboratory. A comprehensive study has been made about the division according to the compositions and properties of soil humus with mathematical method. During the analysis the ratio of humic acid and fulvic acid (HA / FA), two extinction coefficients (E4, E6), and their ratio (E4 / E6). The result indicates that the boundary is at about 1000 meters above sea level.