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目的研究p53、Tip60及14-3-3ó基因在宫颈癌和宫颈上皮内瘤变(CIN)中的表达及其临床意义。方法选取2010年1月至2012年12月在铜川市人民医院手术或经过活检后存档的石蜡包埋的宫颈癌(40例)、CIN(80例)和慢性宫颈炎(20例)手术标本,采用免疫组织化学SP法检测上述标本组织中p53、Tip60和14-3-3ó的基因表达,并分析它们的临床病理参数及其关系。结果在慢性宫颈炎组织未见p53表达,宫颈癌组织中p53的表达明显高于CIN组织,但14-3-3ó的表达则明显低于CIN(P<0.05)。不同级别的CIN病变,各基因表达差异无统计学意义(P>0.05)。Tip60在宫颈癌、CIN和慢性宫颈炎中均有表达(60.0%~77.5%),但差异无统计学意义(P>0.05)。宫颈癌组织中p53,Tip60和14-3-3ó表达与癌组织分化程度、临床分期和淋巴结转移无关(P>0.05)。结论p53、Tip60和14-3-3ó在宫颈癌组织中呈高表达,提示这些基因可能在宫颈癌的发生、发展过程中发挥重要作用。
Objective To study the expression and clinical significance of p53, Tip60 and 14-3-3 o genes in cervical carcinoma and cervical intraepithelial neoplasia (CIN). Methods Surgical specimens of paraffin-embedded cervical cancer (40 cases), CIN (80 cases) and chronic cervicitis (20 cases) were collected from January 2010 to December 2012 in Tongchuan People’s Hospital after operation or biopsy. The expression of p53, Tip60 and 14-3-3 ¢ ó in the specimens were detected by immunohistochemical SP method, and their clinicopathological parameters and their relationship were analyzed. Results There was no p53 expression in chronic cervicitis tissues. The expression of p53 in cervical cancer tissues was significantly higher than that in CIN tissues, but the expression of 14-3-3 o was significantly lower than that of CIN (P <0.05). Different levels of CIN lesions, the gene expression difference was not statistically significant (P> 0.05). Tip60 expression in cervical cancer, CIN and chronic cervicitis (60.0% ~ 77.5%), but the difference was not statistically significant (P> 0.05). The expression of p53, Tip60 and 14-3-3 ¢ ñ in cervical cancer tissues had no correlation with the differentiation degree, clinical stage and lymph node metastasis (P> 0.05). Conclusions The expression of p53, Tip60 and 14-3-3 ¢ ñin cervical cancer tissues is high, suggesting that these genes may play an important role in the development of cervical cancer.