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[目的]希金斯炭疽菌可引起菜心、萝卜等十字花科植物的炭疽病,给经济产生巨大损失。目前,炭疽病菌对苯并咪唑类杀菌剂所产生的抗性问题引起了学者广泛关注,有待于开发新的作用靶标的化学药剂。腺苷酸环化酶(AC)在G蛋白信号传导途径上发挥着重要作用。[方法]基于酿酒酵母中Srv2序列,通过Blastp比对及关键词搜索,并利用SMART进行保守结构域分析,同时,通过对该菌中AC相关蛋白序列进行理化性质、信号肽、跨膜结构域等分析。[结果]明确该菌含有2个与Srv2同源的AC相关蛋白Cg Cap1、Cg Cap2,就理化性质、二级结构等特征方面而言,Cg Cap1、Cg Cap2与Srv2有着较大的差异。此外,通过对Srv2与Cg Cap1、Cg Cap2及其同源序列进行比对分析,发现Cg Cap1、Cg Cap2序列分别与其同源序列及Srv2的C端、N端具有较大的相似性,推测上述序列分别为希金斯炭疽菌AC的C端和N端。[结论]该研究为深入开展该菌AC的研究打下坚实的理论基础。
[Objective] Anthracnose of Higgins can cause anthracnose of cruciferous plants, such as Brassica campestris, radish and so on, which has a huge economic loss. At present, the resistance of anthrax bacteria to benzimidazole fungicides has aroused widespread concern of scholars and needs to be developed into new target chemicals. Adenylate cyclase (AC) plays an important role in the G-protein signaling pathway. [Method] Based on the sequence of Srv2 in Saccharomyces cerevisiae, the Blastp alignment and keyword search were used to analyze the conserved domain of SMv in Saccharomyces cerevisiae. At the same time, the physical and chemical properties, signal peptide and transmembrane domain Other analysis. [Result] Clearly the bacteria contained two AC-related proteins Cg Cap1 and Cg Cap2 which are homologous to Srv2. There are great differences in Cg Cap1, Cg Cap2 and Srv2 in terms of physical and chemical properties, secondary structure and other characteristics. In addition, by comparing and analyzing Srv2 with Cg Cap1, Cg Cap2 and their homologous sequences, it was found that Cg Cap1 and Cg Cap2 sequences have great similarity with their homologous sequences and C-terminal and N-terminal of Srv2 respectively. The sequences were C-terminal and N-terminal of Higgins anthrax AC respectively. [Conclusion] The study laid a solid theoretical foundation for the further study on AC of this strain.